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Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  Beta_CTF_Human

Human APP beta-CTF ELISA Kit

The accumulation of amyloid beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). More than 50 different mutations in the APP gene can cause early-onset Alzheimer disease. Cleavage of APP by beta-secretase results in a beta-C-terminal fragment (beta-CTF). This fragment is further cleaved by gamma-secretase to produce amyloid-beta and soluble APP-beta.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human APP beta-CTF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure human APP beta-CTF protein in cell lysates.

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Features

  • Capable of measuring human APP beta-CTF
  • Sensitivity of 0.02 pmol/L

Applications

  • Measuring human APP beta-CTF protein in cell lysates

Components

Precoated plate: Anti-APP-C Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human
A-beta (N) (82E1) Mouse IgG MoAb Affinity-Purified
0.4 mL x 1
Standard: Human APP beta-CTF synthetic peptide0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.19–12 pmol/L
  • Assay sensitivity: 0.02 pmol/L
  • Assay duration: Overnight
  • Specificity: Human APP beta-CTF; does not cross-react with human sAPP-alpha, sAPP-beta, or sAPP-beta-sw
  • Test specimen type: Cell lysates
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27776A Human APP beta CTF Assay Kit - IBL 96-Well $606.00 *
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