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Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  BACE1

BACE1 ELISA Kit

The accumulation of amyloid beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). More than 50 different mutations in the APP gene can cause early-onset Alzheimer disease. Cleavage by beta- and gamma-secretase (amyloidogenic pathway) leads to production of amyloid-beta and soluble APP-beta. The major beta-secretase enzyme is beta-site APP cleaving enzyme 1 (BACE1), also known as aspartyl protease 2 (ASP2), beta-secretase 1, HSPC104, KIAA1149, membrane-associated aspartic protease 2, and memapsin-2. It is a transmembrane protein that contains two aspartate residues in its active site. Elevated levels of BACE1 have been identified in late-onset sporadic AD.

This kit is a solid phase sandwich ELISA that uses two antibodies that are highly specific to BACE1; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure human, mouse, or rat BACE1 protein in brain tissue extracts or cultured cell lysates.

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Features

  • Capable of measuring human, mouse, and rat BACE1
  • Sensitivity of 0.16 ng/mL

Applications

  • Measuring BACE1 in brain tissue extracts or cultured cell lysates

Components

Precoated plate: Anti-BACE1 (N42) Rabbit IgG Affinity-Purified96 well x 1
Labeled antibody (30X): HRP conjugated Anti-BACE1 (C) Rabbit
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Human BACE1 COS-7 Lysat0.5 mL x 2
EIA buffer:30 ml x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 1.56–100 ng/mL
  • Assay Sensitivity: 0.16 ng/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human, mouse, rat BACE1 (native or recombinant)
  • Species cross-reactivity: Reacts with human, mouse, and rat BACE1 (native or recombinant)
  • Test specimen type: Brain tissue extracts or cultured cell lysates
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27752A BACE1 Assay Kit - IBL 96-Well $606.00 *
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