The accumulation of amyloid beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). More than 50 different mutations in the APP gene can cause early-onset Alzheimer disease. Cleavage by beta- and gamma-secretase (amyloidogenic pathway) leads to production of amyloid-beta and soluble APP-beta. The major beta-secretase enzyme is beta-site APP cleaving enzyme 1 (BACE1), also known as aspartyl protease 2 (ASP2), beta-secretase 1, HSPC104, KIAA1149, membrane-associated aspartic protease 2, and memapsin-2. It is a transmembrane protein that contains two aspartate residues in its active site. Elevated levels of BACE1 have been identified in late-onset sporadic AD.
This kit is a solid phase sandwich ELISA that uses two antibodies that are highly specific to BACE1; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure human, mouse, or rat BACE1 protein in brain tissue extracts or cultured cell lysates.