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Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  Amyloid_Beta_Oligomers_Human

Amyloid-beta Oligomers ELISA

The Amyloid-beta Oligomers ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies to human amyloid-beta oligomers; one is precoated onto the Amyloid-beta Oligomers ELISA plate and the other one is HRP-conjugated. This Amyloid-beta Oligomers Assay can be used to measure human amyloid-beta oligomers in EDTA-plasma, cerebrospinal fluid, serum, cell culture media or brain extract. The Amyloid-beta Oligomers ELISA kit detects human amyloid-beta oligomers that contain two or more epitopes in the N-terminus that are recognized by the anti-human amyloid-beta (82E1) mouse monoclonal antibody.

The accumulation of amyloid-beta protein plaques in the cerebral cortex is a hallmark of Alzheimer's disease (AD). Amyloid-beta is produced by the proteolytic cleavage of amyloid precursor protein (APP), which is mediated by the enzymes beta- and gamma-secretase. This cleavage reaction produces a number of peptides ranging from 36 to 43 amino acids in length. Amyloid-beta insoluble protein is the main component of plaques found in the brains of patients with Alzheimer's disease; however, recent research suggests that soluble, oligomeric forms of amyloid-beta are also involved in this disease.

At-A-Glance Documents Images & Data

Features

Cat.# 27725A

  • Capable of measuring human amyloid-beta oligomers
  • Sensitivity: 4.41 pmol/L

Applications

  • Measurement of human amyloid-beta oligomers in EDTA-plasma, cerebrospinal fluid, serum, cell culture media or brain extract

Components

Precoated plate: Anti-Human Amyloid-beta (N) (82E1) Mouse
IgG MoAb, affinity purified
96 Well x 1
Labeled antibody (30X): HRP-conjugated Anti-Human Amyloid-
beta (N) (82E1) Mouse IgG MoAb, affinity purified
0.1 mL x 1
Standard: Human Amyloid-beta(1-16) Dimer0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS 30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1
U-Plate for primary incubation (with a sticker attached):96 Well x 1

Storage

2–8°C

Performance

  • Range of assay: 18.98–1,215 pmol/L
  • Assay sensitivity: 4.41 pmol/L
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human amyloid-beta (1-16) dimer; 0.27% cross-reactivity with amyloid-beta (1-40) peptide
  • Species cross-reactivity: Does not cross-react with mouse or rat amyloid-beta
  • Test specimen type: EDTA-plasma, cerebrospinal fluid, serum, cell culture media or brain extract
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27725A Human Amyloid beta Oligomers (82E1-specific) Assay Kit - IBL 96-Well $725.00
 

 



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