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Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  Alpha2_6-Sialytransferase_ELISA

alpha-2,6-Sialyltransferase ELISA Kits

The accumulation of amyloid beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). More than 50 different mutations in the APP gene can cause early-onset Alzheimer disease. Cleavage by beta- and gamma-secretase (amyloidogenic pathway) leads to production of amyloid-beta. Alpha-2,6-sialyltransferase (ST6Gal I) is a glycosyltransferase involved in the biosynthesis of sugar chains. It is also known as alpha 2,6-ST 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase), ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, ST6N, and SIAT1. Recent studies have shown that ST6Gal I is also cleaved by beta-secretase, both in vitro and in vivo, to produce a peptide known as ST6Gal I (E41 Form).

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human, mouse, and rat ST6Gal I protein (Cat.# 27762) and rat ST6Gal I (E41 form) (Cat.# 27408); one is precoated on the ELISA plate and the other is HRP-conjugated. These kits can be used to measure ST6Gal I in serum, EDTA plasma, or cell culture supernatant.

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Features

  • Capable of measuring human, mouse, and rat ST6Gal I protein (Cat.# 27762) and rat ST6Gal I (E41 form) (Cat.# 27408)
  • Sensitivity of 0.02 ng/mL (Cat.# 27408); 0.20 ng/mL (Cat.# 27762)

Applications

  • Measuring human, mouse, and rat ST6Gal I protein (Cat.# 27762) and rat ST6Gal I (E41 form) (Cat.# 27408) in serum, EDTA plasma, or cell culture supernatant

Components

Precoated plate: Anti-ST6Gal I Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-ST6Gal I Rabbit IgG
Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant rat ST6Gal I protein0.5 mL x 2
EIA buffer:30 mL x 1
Solution for labeled antibody:12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.31–20 ng/mL (Cat.# 27408); 1.09–70 ng/mL (Cat.# 27762)
  • Assay Sensitivity: 0.02 ng/mL (Cat.# 27408); 0.20 ng/mL (Cat.# 27762)
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Rat ST6Gal I (E41 form) (Cat.# 27408); human, mouse, rat ST6Gal I (Cat.# 27762)
  • Species cross-reactivity: n/a
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatant
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Contents Size Price Units Select
27762A alpha2, 6-Sialyltransferase Assay Kit - IBL 96-Well $606.00 *
27408A Rat alpha2, 6-Sialyltransferase (E41 Form) Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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