Takara
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  Alpha2_6-Sialytransferase_APL1-beta25_27_28

Human APL1-beta ELISA Kits

Amyloid beta (A4) precursor-like protein 1 (APLP1) is a member of the highly conserved amyloid precursor protein (APP) family. It is also known as amyloid precursor-like protein 1 and amyloid-like protein 1. APLP1 is a membrane-associated glycoprotein that is cleaved by beta-secretase (BACE) and gamma-secretase in a similar fashion to APP itself. The cleavage products are secreted into cerebrospinal fluid like those derived from APP, but they do not accumulate in the cerebral cortex. Three cleavage APLP1-derived Abeta-like peptides (APL1-beta) are known: APL1-beta25, APL1-beta27, and APL1-beta28. These peptides have been suggested as novel biomarkers for Alzheimer's disease.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human APL1-beta25, APL1-beta27, and APL1-beta28; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human APL1-beta25, APL1-beta27, and APL1-beta28 in cerebrospinal fluid or cell culture supernatants.

At-A-Glance Documents

Features

  • Capable of measuring human APL1-beta25, APL1-beta27, and APL1-beta28
  • Sensitivity: 2.49 pg/mL (Cat.# 27737); 2.72 pg/mL (Cat.# 27738); 3.18 pg/mL (Cat.# 27739)

Applications

  • Measuring human APL1-beta25, APL1-beta27, and APL1-beta28 in cerebrospinal fluid or cell culture supernatants

Components

Precoated plate: Anti-APL1-beta (25/27/28)
Rabbit IgG Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-
Human APL1-beta (N) Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Human APL1-beta (25/27/28)0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 9.38–600 pg/mL (Cat.# 27737); 4.69–300 pg/mL (Cat.# 27738); 46.88–3000 pg/mL (Cat.# 27739)
  • Assay sensitivity: 2.49 pg/mL (Cat.# 27737); 2.72 pg/mL (Cat.# 27738); 3.18 pg/mL (Cat.# 27739)
  • Assay duration: Overnight
  • Specificity: Each ELISA kit shows <0.2% cross-reactivity with the other APL1-beta isoforms
  • Test specimen type: Cerebrospinal fluid or cell culture supernatants
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27737A Human APL1beta25 Assay Kit - IBL 1 x 96-Well Plate $874.00
27738A Human APL1beta27 Assay Kit - IBL 1 x 96-Well Plate $874.00
27739A Human APL1beta28 Assay Kit - IBL 1 x 96-Well Plate $874.00
 

 



Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions