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Products >  Cell_Biology >  Alzheimers_Disease >  ELISA_Kits >  APP770_Human

Human APP770 ELISA Kit

The accumulation of amyloid beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). More than 50 different mutations in the APP gene can cause early-onset Alzheimer disease. Cleavage by beta- and gamma-secretase (amyloidogenic pathway) leads to production of amyloid-beta and soluble APP-beta, while cleavage by alpha-secretase (nonamyloidogenic pathway) results in a soluble form of APP, sAPP-alpha. Amyloid-beta proteins that build up in brain parenchyma are mostly produced from APP expressed in neurons, APP695. A different form of APP, APP770, has been detected in cerebrovascular endothelial cells. Amyloid-beta derived from APP770 can accumulate on cerebrovascular walls.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human APP770 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure human APP770 protein in EDTA plasma, cerebrospinal fluid, or cell culture supernatant.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human APP770 protein
  • Sensitivity of 0.03 ng/mL

Applications

  • Measuring human APP770 protein in EDTA plasma, cerebrospinal fluid, or cell culture supernatant

Components

Precoated plate: Anti-Human APP OX2 (351) Rabbit IgG
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human APP
(R101A4) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant human APP7700.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.10–6.2 ng/mL
  • Assay sensitivity: 0.03 ng/mL
  • Assay duration: Overnight
  • Specificity: Human APP770; does not cross-react with human APP695 or APP751
  • Test specimen type: EDTA plasma, cerebrospinal fluid, or cell culture supernatant
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27736A Human APP770 Assay Kit - IBL 96-Well $750.00
 

 



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