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Products >  Cell_Biology >  Alzheimers_Disease >  Antibodies >  sAPP_alpha_Mouse_Monoclonal

APP Antibody

Takara Bio's Amyloid Precursor Protein (APP) Antibody products are affinity-purified IgG antibodies that recognize the C-terminus of human soluble APP-alpha (Cat.# 11088) and soluble APP-beta-Swedish (Cat.# 10321). The APP Antibody products are raised in mouse using synthetic peptides and can be applied to Western blot (WB) and immunohistochemical (IHC) detection (Cat.# 10321 only) as well as immunoprecipitation (IP) (Cat.# 11088 only) of human sAPP-alpha and sAPP-beta-sw.

The accumulation of amyloid beta protein plaques in the cerebral cortex in Alzheimer's disease (AD) is one of the outcomes of APP proteolytic cleavage. More than 50 different APP gene mutations can cause early-onset Alzheimer disease. Cleavage by beta- and gamma-secretase (amyloidogenic pathway) leads to production of the pathologic amyloid-beta protein as well as soluble APP-beta, while cleavage by alpha-secretase (nonamyloidogenic pathway) results in the soluble form of APP (sAPP-alpha). Different mutations in the APP gene can give rise to variants of both sAPP-alpha and sAPP-beta.

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Features

Cat.#: 10321A, 10321B
  • Name: Human sAPP-beta-sw
  • Antigen: Human sAPP-beta-sw C-terminal synthetic peptide
  • Application: IHC, WB
  • Specificity: Does not cross-react with sAPP-beta-wt or full-length APP-sw/APP-wt.
  • Entrez Gene ID: 351

Cat.#: 11088A, 11088B
  • Name: Human sAPP-alpha
  • Antigen: Human sAPP-alpha C-terminal synthetic peptide
  • Application: WB, IP
  • Specificity: Does not cross-react with sAPP-beta or full-length APP; cross-reacts with mouse/rat sAPP-alpha
  • Entrez Gene ID: 351

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • IHC: 2 µg/mL
  • WB: 2–5 µg/mL
  • IP: 3 µg/mL

Storage

  • Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C

Form

  • Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3
  • To use, reconstitute each 10 ug vial in 100 ul H2O and each 100 ug vial in 1.0 mL H2O (final concentration, 100 µg/mL). 

Alternative names

AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTF-gamma




 
 
Products
Cat. # Product Contents Size Price Units Select
10321A Anti-Human sAPP beta-sw (6A1) Mouse IgG MoAb 10 ug $113.00 *
10321B Anti-Human sAPP beta-sw (6A1) Mouse IgG MoAb 100 ug $499.00
11088A Anti-Human sAPP alpha(2B3) Mouse IgG MoAb 10 ug $113.00 *
11088B Anti-Human sAPP alpha(2B3) Mouse IgG MoAb 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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