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Products >  Cell_Biology >  Alzheimers_Disease >  Antibodies >  BACE1_Human

Human BACE1 Antibody

The Human BACE1 Antibody products are affinity-purified IgG antibodies that recognize human BACE1 protein. The BACE1 antibodies are raised in rabbit using synthetic peptides and can be applied to Western blot (WB) and immunohistochemistry (IHC) detection as well as immunoprecipitation (IP) of human BACE1 protein.

The accumulation of amyloid beta protein in the cerebral cortex in Alzheimer's disease (AD) is one of the outcomes of the aberrant proteolytic cleavage of amyloid precursor protein (APP). Aberrant cleavage can occur due to the more than 50 different mutations that have been documented in the APP gene, and which are also associated with early-onset Alzheimer's disease.

Cleavage of APP occurs via beta- and gamma-secretase (through the amyloidogenic pathway), leading to the production of amyloid-beta and soluble APP-beta. The major beta-secretase enzyme is beta-site APP cleaving enzyme 1 (BACE1), also known as aspartyl protease 2 (ASP2), beta-secretase 1, HSPC104, KIAA1149, membrane-associated aspartic protease 2 and memapsin-2. BACE1 is a transmembrane protein that contains two aspartate residues in its active site. Elevated levels of BACE1 have been correlated with late-onset sporadic AD.

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Features

Cat.#: 18711A, 18711B

  • Target Name: Human BACE1 protein
  • Antigen: Human BACE1 C-terminal synthetic peptide
  • Application: WB, IHC, IP
  • Specificity: Cross-reacts with mouse and rat BACE1
  • Target Entrez Gene ID: 23621

  • Cat.#: 28051A, 28051B

  • Target Name: Human BACE1 protein
  • Antigen: Human BACE1 synthetic peptide (RLPRETDEEPEEPGRR)
  • Application: IHC, WB
  • Specificity: Cross-reacts with mouse and rat BACE1
  • Target Entrez Gene ID: 23621
  • Applications

    Recommended BACE1 antibody concentration (Note: optimal concentration should be determined empirically):

    • Western blot: 2 µg/mL (Cat.# 18711)
    • Immunohistochemistry: 5 µg/mL (Cat.# 18711); 1-5 µg/mL (Cat.# 28051)
    • Immunoprecipitation: 2 µg/mL (Cat.# 18711)

    Storage

    Lyophilized: stable up to 5 years at 2–8°C. In solution: stable up to 2 years at –20°C.

    Form

    Lyophilized from BACE1 antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in H2O (final concentration: 100 µg/mL).

    Alternative names

    Aspartyl protease 2, ASP2, beta-secretase 1, HSPC104, KIAA1149, membrane-associated aspartic protease 2, memapsin-2




     
     
    Products
    Offers Cat. # Product Contents Size Price Units Select
    28051A Anti-BACE1 (42) Rabbit IgG Affinity Purify 10 ug $113.00 *
    28051B Anti-BACE1 (42) Rabbit IgG Affinity Purify 100 ug $499.00
    18711A Anti-Human BACE1 (C) Rabbit IgG Affinity Purify 10 ug $113.00 *
      18711B Anti-Human BACE1 (C) Rabbit IgG Affinity Purify 100 ug $499.00
    * You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
     

     

     
     

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