Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Alzheimers_Disease >  Antibodies >  Alpha2_6-Sialyltransferase

Alpha 2,6-Sialyltransferase Antibody

The Alpha 2,6-Sialyltransferase Antibody or ST6Gal-I Antibody is affinity-purified to recognize ST6Gal-I protein. The Alpha 2,6-Sialyltransferase Antibody is raised in rabbit using synthetic peptides and can be applied to Western blot (WB) detection of ST6Gal-I protein.

The accumulation of amyloid beta plaques in the cerebral cortex during Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). Over 50 different mutations in the APP gene have been found to increase the chances of amyloid beta plaque generation, leading to early-onset Alzheimer disease. Cleavage of mutated APP by beta- and gamma-secretase (amyloidogenic pathway) leads to production of pathologic, insoluble amyloid-beta deposits as well as non-pathologic, soluble APP-beta (sAPP-beta), while cleavage by alpha-secretase (nonamyloidogenic pathway) results in only sAPP-alpha.

Recent studies have also identified that the glycosyltransferase ST6 beta-galactoside alpha-2,6-sialyltransferase (ST6Gal-I) is cleaved by beta-secretase (BACE1)1. ST6Gal-I catalyzes the transfer of an alpha 2,6-linked sialic acid to the N-glycans of certain receptors. It is also known as alpha-2,6-ST1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase), beta-galactoside alpha-2,6-sialyltransferase 1, sialyltransferase 1, 1ST6N and SIAT1. In humans, BACE1 cleavage of ST6Gal-I generates alpha-2,6-sialyltransferase (E44 form), while in rats it generates alpha-2,6-sialyltransferase (E41 form).

  At-A-Glance   Documents   Images & Data

Features

Cat.# 18983A, 18983B
  • Name: Rat ST6Gal-I protein (E41)
  • Antigen: Rat ST6Gal I (E41) synthetic peptide
  • Application: WB
  • Specificity: Does not cross-react with human or mouse ST6Gal I
  • Entrez Gene ID: 25197

Cat.# 18985A, 18985B

  • Name: Human/Mouse/Rat ST6Gal-I protein
  • Antigen: ST6GalI C-terminal synthetic peptide
  • Application: WB
  • Specificity: Reacts with human, mouse and rat ST6Gal I
  • Entrez Gene ID: 6480, 20440, 25197

Cat.# 28047A, 28047B

  • Name: Human/Mouse/Rat ST6Gal-I protein
  • Antigen: ST6Gal I C-terminal synthetic peptide
  • Application: WB
  • Specificity: Reacts with human, mouse and rat ST6Gal I (cleaved and full-length)
  • Entrez Gene ID: 6480, 20440, 25197

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • WB: 1–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

References

1. Kitazume, S. et al. (2001) Proc. Natl. Acad. Sci. USA. 98(24):13554–13559.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute each 10 ug vial in 100 ul H2O and each 100 ug vial in 1.0 mL H2O (final concentration, 100 µg/mL).

Alternative names

ST6Gal-I

alpha-2,6-ST1

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1

sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)

beta-galactoside alpha-2,6-sialyltransferase 1

sialyltransferase 1

1ST6N

SIAT1




 
 
Products
Cat. # Product Contents Size Price Units Select
18983A Anti-Rat alpha2, 6-Sialyltransferase (E41) Rabbit IgG Affinity Purify 10 ug $113.00 *
18983B Anti-Rat alpha2, 6-Sialyltransferase (E41) Rabbit IgG Affinity Purify 100 ug $499.00
18985A Anti-alpha2, 6-Sialyltransferase (C) Rabbit IgG Affinity Purify 10 ug $113.00 *
18985B Anti-alpha2, 6-Sialyltransferase (C) Rabbit IgG Affinity Purify 100 ug $499.00
28047A Anti-alpha2, 6-Sialyltransferase (M2) Rabbit IgG Affinity Purify 10 ug $113.00 *
28047B Anti-alpha2, 6-Sialyltransferase (M2) Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions