Mouse IgG EIA Kit is a quantification kit that uses a mouse IgG-specific polyclonal antibody. It can be used to measure IgG in mouse blood, test the quality of drugs or research reagents that use a monoclonal antibody, and monitor antibody production of hybridoma cells. This kit comes with a ready-to-use plate coated with solid-phase capture antibody. Its usage and dosage are designed to allow the detection of mouse immunoglobulin with good sensitivity and reproducibility within a short time–only 60 minutes for the entire assay.
Immunoglobulins (Ig) are antibody proteins that play a key role in antibody-mediated immunity. There are five classes of immunoglobulins (IgG, IgA, IgM, IgE and IgD) present in animals, with IgG accounting for 80% of the immunoglobulins circulating in blood. IgG levels vary greatly depending on the immune response; however, IgG is generally present in the blood at 3–10 mg/mL. This is a high enough concentration that IgG can be detected without using a high sensitivity ELISA assay.
Monoclonal antibodies are uniform immunoglobulins produced by hybridoma cells. Hybridomas are a fusion between antibody-forming cells and tumor cells grown in culture. The wide-spread use of monoclonal antibody production technology has allowed the isolation of high-purity immunoglobulins, which have been used as reagents for research and as the main component in some therapeutics. Scientists that work with hybridoma cells monitor immunoglobulin production by detecting mouse IgG in the hybridoma culture supernatant. Unlike IgG levels in blood, IgG is present in hybridoma supernatants at low levels and a high sensitivity assay is required for detection.
