One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for real time one-step RT-PCR using TaqMan probe1,2 for detection. In this kit, RT-PCR can be performed in a single tube, simplifying the protocol and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis after PCR. The sensitivity of the enzymes in this kit enables detection of very small amounts of RNA, including RNA virus samples.
This kit uses PrimeScript RTase, an RNase H Minus MMLV-based reverse transcriptase which displays excellent extension and fast full-length cDNA synthesis, and TaKaRa Ex Taq HS high efficiency hot start PCR enzyme, both optimized for one step RT-PCR.
Applicable real time PCR instruments
- Thermal Cycler Dice Real Time System (TaKaRa Cat.# TP800)3
- ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System (Applied Biosystems)
- LightCycler (Roche Diagnostics)
- Smart Cycler System4/Smart Cycler II System4 (Cepheid), and others
1TaqMan is a registered trademark of Roche Molecular Systems Inc.
2Use One Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time) for real time one-step RT-PCR using SYBR Green I for detection.
3Thermal Cycler Dice Real Time System is not sold worldwide. Please check for availability in your region.
4Smart Cycler is a registered trademark of Cepheid.