Currently, detection and identification of Campylobacter is done by the culture method. This method is laborious due to the slow growth of the bacteria which takes at least 2 days for pre-culture and then several days for identification of the species type. Therefore, a week is required for definite identification of the Campylobacter species.
A rapid detection and identification method for Campylobacter using PCR has been developed by Takara Bio. This PCR detection and typing kit is designed to specifically and rapidly detect and identify the 3 types of Campylobacter species. The kit uses PCR to detect targets of the B subunit gene (cdtB gene) or C subunit gene (cdtC gene) of the Cytolethal distending toxin (cdt genes). In the Campylobacter species, there are occasionally strains which have deletions or mutations on their cdtB gene or cdtC gene. In order to increase the detection accuracy of this kit, detection of both the cdtB and cdtC genes is performed. The kit uses Takara’s high performance enzyme, Ex Taq Hot Start Version, which allows high sensitivity detection of the target genes. TaKaRa’s Ex Taq HS decreases mis-priming during preparation of reaction solutions and non-specific amplifications derived from primer dimers.
This kit was designed by TaKaRa through the cooperation with Osaka Prefecture University and Fuso Pharmaceutical Industries, Ltd.