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Products >  PCR_Products >  Pathogen_Detection_and_Screening_Kits >  Food_Pathogen_Detection >  Campylobacter_PCR_Detection_and_Typing_Kit

Campylobacter PCR Detection and Typing Kit

Recently the incidence of food poisoning caused by Campylobacter has become more prevalent than in previous years. Campylobacter is a gram-negative, microaerophilic, spiral-shaped, non-spore forming bacteria. There are three species in the Campylobacter genus: Campylobacter jejuni (C. jejuni), Campylobacter coli (C. coli) and Campylobacter fetus (C. fetus). It is one of the foremost causes of human gastroenteritis in the world. Approximately 90% of the pathogenic bacteria for the gastroenteritis are C. jejuni and a small percent are C. coli. Campylobacter fetus (C. fetus) is isolated from septicemic or meningitidis specimens, and are considered pathogenic bacteria for these illnesses. The symptoms are different depending on the Campylobacter species, therefore, it is very important not only to detect the presence of the bacteria but also to identify the species of the bacterium.

Currently, detection and identification of Campylobacter is done by the culture method. This method is laborious due to the slow growth of the bacteria which takes at least 2 days for pre-culture and then several days for identification of the species type. Therefore, a week is required for definite identification of the Campylobacter species.

A rapid detection and identification method for Campylobacter using PCR has been developed by Takara Bio. This PCR detection and typing kit is designed to specifically and rapidly detect and identify the 3 types of Campylobacter species. The kit uses PCR to detect targets of the B subunit gene (cdtB gene) or C subunit gene (cdtC gene) of the Cytolethal distending toxin (cdt genes). In the Campylobacter species, there are occasionally strains which have deletions or mutations on their cdtB gene or cdtC gene. In order to increase the detection accuracy of this kit, detection of both the cdtB and cdtC genes is performed. The kit uses Takara’s high performance enzyme, Ex Taq Hot Start Version, which allows high sensitivity detection of the target genes. TaKaRa’s Ex Taq HS decreases mis-priming during preparation of reaction solutions and non-specific amplifications derived from primer dimers.

This kit was designed by TaKaRa through the cooperation with Osaka Prefecture University and Fuso Pharmaceutical Industries, Ltd.

At-A-Glance Documents

Components

(for 100 reactions1, 50 µL PCR)

Premix Ex Taq HS2 (2X conc.) 5 x 500 µL
cdtB Primer Mix 250 µL
cdtC Primer Mix 250 µL
dH2O 3 x 1 mL
C. jejuni Positive Control 150 µL
C. coli Positive Control 150 µL
C. fetus Positive Control 150 µL

150 reactions for cdtB gene detection and 50 reactions for cdtC gene detection.

22X concentration of PCR reaction reagent which contains TaKaRa Ex Taq HS, Reaction Buffer, and dNTP mixture. As the activity might be reduced by repeated freeze-thaws, aliquot 25 µL into separate tubes and store at –20°C after thawing.

Storage

–20°C

References

  1. Worada Samosornsuk, et al. (2007) Microbiol. Immunol., 51 (9), 909-917.
  2. Asakura, M., et al. (2007) Microb. Pathog., 42, 174-183.



 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR134A Campylobacter (cdt gene) PCR Detection and Typing Kit 100 Rxns $702.00 License Statements
 

 


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