Components
RR003A |
| TaKaRa Premix Ex Taq* | 6 x 500 µL |
*Contains TaKaRa Ex Taq, Ex Taq Buffer, 4 mM MgCl and dNTPs.
Storage
–20°C. Avoid repeated freeze-thaw and vigorous stirring of product. Once thawed, aliquot product into PCR tubes and store at –20°C.
PCR Performance
Enzyme performance is confirmed by successful amplification of a 20 kb lambda DNA template. PCR amplification of a single copy gene (Beta-globin) by PCR is confirmed using human genomic DNA as the template (amplified fragment: 17.5 kb).
PCR Products
PCR products generated with Ex Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors.
References
1. Barnes, W.M. (1994) Proc. Natl. Acad. Sci. USA 91:2216-2220.
2. Cheng, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5695-5699.
3. Cheng, S. et al. (1994) Nature Genet. 7:350-351.
4. Cheng, S. et al. (1994) Nature 369:684-685.
5. Saiki, R.K. et al. (1988) Science 239:487-491.
6. Scharf, S.J. et al. (1986) Science 233:1076-1078.
7. Gyllensten, U.B. & Erlich, H.A. (1988) Proc. Natl. Acad. Sci. USA 85:7652-7656.
8. Kawasaki, E.S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:5698-5702.
9. Lawyer, F.C. et al. (1988) J. Biol. Chem. 264:6427-6437.
