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Products >  PCR_Products >  High_Performance_PCR >  LA_Taq_Hot-Start_Version

LA Taq DNA Polymerase, Hot-Start Version

TaKaRa LA Taq DNA Polymerase, Hot-Start Version consists of TaKaRa LA Taq polymerase plus a monoclonal antibody specific to Taq polymerase. This thermostable enzyme retains all of the high performance features of TaKaRa LA Taq polymerase while increasing reaction specificity and reducing background. TaKaRa LA Taq DNA Polymerase is optimized for long range PCR, enabling synthesis of products up to 30 kb for genomic DNA and 48 kb for lambda DNA templates. The polymerase also has 6.5X higher fidelity than Taq DNA Polymerase. The use of LA Taq polymerase requires little optimization and provides greater product yield compared to other manufacturers' long range PCR enzymes due to a robust enzyme-buffer system. Hot start PCR amplification reactions can be assembled at room temperature using TaKaRa LA Taq DNA Polymerase, Hot-Start Version.

At-A-Glance Documents Images & Data Resources

Features

  • 6.5X higher fidelity amplifications compared with conventional Taq DNA Polymerase.
  • Less optimization and greater yield due to a robust enzyme-buffer system.
  • Reduced background and increased reaction specificity.

Applications

  • Long range PCR to amplify products up to 48 kb
  • Multiplex PCR
  • Amplification prior to sequencing with next-generation sequencing (NGS) platforms such as Illumina HiSeq, Illumina Genome Analyzer (GA), and pyrosequencing using Roche 454 GS FLX
 

Components

RR042A

TaKaRa LA Taq DNA Polymerase, Hot Start Version  125 U (5 U/µL)*
10X LA PCR Buffer II (Mg2+)25 mM
dNTP Mixture (2.5 mM each dNTP)400 µL
*Protocol recommends the use of 2.5 U per 50 µL reaction.

Storage

–20°C. Avoid repeated freeze-thaw of TaKaRa LA Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.

PCR Products

PCR products generated with TaKaRa LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.

PCR Performance

Performance confirmed by successful amplification of a 35 kb lambda DNA template and a 17.5 kb human genomic DNA. Inhibition of TaKaRa LA Taq activity by the antibody is confirmed to be >90% following enzyme incubation at 55°C for 10 min.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR042A TaKaRa LA Taq® DNA Polymerase Hot-Start Version 125 Units $178.00 License Statements
RR042B TaKaRa LA Taq® DNA Polymerase Hot-Start Version 500 Units $656.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
4030 dNTP Mixture 3.2 µmol Each/1.25 mL $57.00
 

 


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