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Products >  Molecular_Biology >  Nucleic_Acids >  Linkers >  Phosphorylated_Linkers

Phosphorylated Linkers

Phosphorylated linkers are cloning reagents that contain a phosphate group at their 5'-end, enabling them to be used directly in linker ligation during cloning experiments. The in-frame cloning of a target gene into a vector can be easily performed by using a phosphorylated linker that results in the desired reading frame.

  At-A-Glance   Images & Data

Features

Sequence Information

Linker, pSma I (6 mer)pd(CCCGGG)

Purity

  • Confirmed by HPLC
  • Biological activity confirmed by self-ligation and restriction enzyme cleavage

Storage

–20°C. Product ships at room temperature.

Notes

These products are single-stranded oligonucleotides. For use in subsequent reactions, dissolve in a buffer such as TE, incubate at 70°C for 10 minutes and anneal by cooling slowly.

Form

Lyophilized

References

1. Smith, D.I. et al. (1976) Nucleic Acids Res. 3:343-353.

2. Brown, N.L. & Smith, M. (1976) FEBS Letters 65:284-287.

3. Roberts, R.J. (1985) Nucleic Acids Res. 13:r165-r200.




 
 
Products
Cat. # Product Size Price Units Select
4685A Linker, Sma I 10,000 pmol $75.00
 

 

 
 

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