Advantage® cDNA Polymerase Mix and PCR Kits

Advantage cDNA Polymerase was designed for improved performance and reliability in the amplification of cDNA targets of up to 10 kb. Benefits over native Taq DNA Polymerase include greater efficiency and sensitivity, amplification of longer products, built-in hot start, and increased fidelity.

Advantage cDNA PCR Kits contain Advantage cDNA Polymerase Mix—an enzyme blend consisting of an N-terminal deletion mutant of Taq DNA Polymerase (1, 2), a proofreading enzyme, and TaqStart® Antibody for automatic hot start PCR. The kits also come with an optimized buffer, dNTP mix, and a control primers and template.

Our Advantage cDNA products have been optimized to produce high yields and long reads. Compared to regular Taq polymerase, Advantage cDNA Polymerase Mix provides more efficient amplification over a wide range of template concentrations (Figure 1). In addition, the optimal range of Mg²⁺ concentration is broader for Advantage cDNA Polymerase Mix than it is for most other enzymes, eliminating the need to spend time optimizing reaction conditions.

This enzyme mix has also been optimized for high fidelity PCR in our Advantage HF PCR Kits, and for complex templates in our Advantage GC cDNA Polymerase Mixes and Kits.