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Yeast Media Bulk Packs - YPD and SD

 
 
 
 

User Manuals

 
 
 
 

Product Literature

 
These high-quality yeast growth media and dropout supplements can be used for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants. They are ideal for use with Matchmaker™ Systems and other yeast one- or two-hybrid systems.
 

YPD Medium

YPD Medium is a blend of peptone, yeast extract, and dextrose in optimal proportions for growing most Saccharomyces cerevisiae strains. This medium is available with or without agar premixed in the medium.
 

YPDA medium

YPDA is the same as YPD but with the addition of an adenine hemisulfate supplement to a final 1X concentration of 120 mg/L. The adenine supplement prevents yeast strains harboring ADE1 and ADE2 mutations (e.g. Y2HGold, AH109, and Y187) from turning pink/red after extended growth. 
 

Minimal SD Bases

Minimal synthetic defined (SD) bases include a yeast nitrogen base, ammonium sulfate, and a carbon source, either dextrose (Minimal SD Base) or galactose and raffinose (Minimal SD Agar Base Gal/Raf). Dropout (DO) supplements can be added to the Minimal SD Base to make a synthetic, defined medium lacking the specified nutrients. The SD bases are available with or without agar premixed in the medium. Each package of Minimal SD Base makes 10 L of medium. Each package of Minimal SD Base Gal/Raf makes 5 L.
 

Dropout Supplements (Click here for more information)

Dropout (DO) supplements provide various mixtures of amino acids and nucleosides to add to the Minimal SD Bases to make a defined minimal medium. In each Dropout mix, certain amino acids have been “dropped out”, for example -Leu/-Trp DO supplement contains every essential amino acid except for leucine and tryptophan. DO Supplements may also be added to the minimal E. coli medium M9 for minimal selection in E. coli. Each package will make at least 10 L of medium.
 
 
 
 

Figure 1. Clontech Bulk Media.