Xfect™ Transfection Reagent

An Innovative Transfection Reagent

Are you looking for a highly efficient transfection reagent that doesn't kill your cells? After a screen of more than 2,300 polymers, we bring you Xfect, the first in a new generation of novel transfection reagents from Clontech. When Xfect is combined with plasmid DNA, it creates biodegradable nanoparticles that provide superior transfection results in a wide variety of mammalian cell types, without the high cytotoxicity of competing transfection reagents.

High Efficiency & Low Toxicity

Effective, nontoxic DNA transfer is a vital first step in studies of gene expression and function. Because Xfect allows you to apply higher amounts of the DNA+Reagent complex without killing the cells, it results in greater numbers of transfected and viable cells than the most popular competitor reagent (Figure 1).

Excellent for Most Cell Types, Even Difficult-to-Transfect Cells

Xfect transfects the following 5 most commonly transfected cell lines with very high efficiency:

• 293T             97% efficiency
• HEK-293     93% efficiency
• HeLa            85% efficiency
• NIH/3T3       82% efficiency
• CHO             83% efficiency

Our customers have indicated via survey that they have successfully used Xfect to transfect a wide variety of cells and cell lines (Table I).

A Simple Protocol

For ease of use, transfections can be carried out entirely in the presence of serum. The Xfect protocol requires few steps and minimal optimization (Figure 2).

Share Without Worry - Convenient 2-pack Format

Xfect is packaged in convenient, separable kits, so if you purchase the 100 reaction kit you can share with your less-than-careful colleagues without fear. Simply give them half of the kit and hide yours!