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Aureobasidin A

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Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic (Figure 1), which is isolated from the filamentous fungus Aureobasidium pullulans R106 and toxic to yeast at low concentrations (0.1–0.5 μg/ml). Sensitive fungi species include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida glabrata, Aspergillus nidulans and A. niger.

AbA inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, that is expressed from the AUR1 gene (1, 2). Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. It is this gene that is used in Matchmaker Gold systems as a reporter for protein interactions.

Perfect Reporter for Yeast Two-Hybrid Studies

AbA selection virtually eliminates the high numbers of background colonies that often plague low-stringency primary screens that use nutritional markers alone (e.g. HIS3). Because AbA actually kills sensitive yeast cells, rather than merely retarding their growth, AbA-based selection greatly favors the growth and identification of genuinely positive clones. In general practice, a high percentage of clones that emerge from low-stringency primary screens using AbA selection alone, are subsequently verified on high-stringency secondary screens that select for all four Matchmaker Gold reporters (AUR1-C, HIS3, ADE2 and MEL1).

References

Takesako, K. et al. (1993) J. Antibiot. (Tokyo) 46(9):1414–20.
Hashida-Okado, T. et al. (1996) Mol. Gen. Genet. 251(2):236–244.

Product Name	Size	Catalog Number	Price	Add to Cart	Notice to Purchaser
Aureobasidin A	1 mg	630466	$167.00 USD
Aureobasidin A	10 mg	630499	$378.00 USD