Matchmaker™ Mammalian Two-Hybrid Assay Kits

Matchmaker Mammalian Two-Hybrid Assay Kits allow you to test protein-protein interactions in transfected mammalian cells by using a standard CAT assay (chloramphenicol acetyltransferase; Cat. No. 630301) or a SEAP assay (secreted alkaline phosphatase; Cat. No. 630305).

Because these two-hybrid assays are performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.

The Matchmaker Mammalian Two-Hybrid Assays are based on the same principle as the Matchmaker Two-Hybrid System—two fusion proteins are expressed that contain either a DNA binding domain or an activation domain. If the proteins interact, transcription of the CAT or SEAP reporter gene is activated from the pG5 plasmid (Figure 1). The interaction between the two proteins of interest can then measured using any standard CAT or SEAP assay (e.g. Great EscAPe™ SEAP Chemiluminescence Kit 2.0; Cat. No. 631736)