Adeno-X™ Maxi Purification Kit
Purify adenovirus quickly and easily with our Adeno-X Maxi Purification Kits! These kits are chromatography-based systems that enable you to purify amplified adenovirus directly from the cell pellet in less than 1.5 hours. As if this weren’t easy enough, a syringe pump can make your preps even easier — ask your Sales Representative for information on how to receive a free pump with your purchase!
Our Adeno-X Maxi Purification Kits have a wide range of viral capacities, allowing you to scale up or down without difficulty. The kit delivers as many as 1 x 1012 adenoviral particles, and provides all of the buffers and materials necessary for purification. This same technology is also available in our convenient Adeno-X Mega Purification Kits.
Unsure Which Kit to Use?
| How much lysate do I have? | Which kit do I need? |
| 25 x 15 cm plates | Adeno-X Mega Purification Kit |
| 5 x 15 cm plates | Adeno-X Maxi Purification Kit |
Simple Chromatographic Procedure
Our protocol contains no ultracentrifugation steps. Instead, adenovirus is purified chromatographically using a unique membrane that selectively binds adenoviral particles. The membrane is housed in small, single-use cartridge that fits securely on standard size BD Luer-Lok™ syringes. For added convenience, the syringe-filter assembly comes completely preassembled and ready to use. The adenovirus-containing medium is brought into the syringe through tubing attached to a one-way valve, and is pushed out through the purification cartridge. As virus-containing medium passes through the purification cartridge, the adenoviral particles adhere to the membrane and are effectively removed from the solution. The bound particles are then washed and eluted (Figure 1).
Achieve High Titers of High Quality Virus
The high titer and purity achieved by our Adeno-X Purification Kits is comparable to that achieved by CsCl density gradient centrifugation, with viral particle to infectious unit ratios as low as 20:1. Unlike techniques involving CsCl, our method is safe, and requires no advanced training or expensive equipment—only simple manipulations are required to produce consistently high yields of purified adenovirus.
Figure 1. Overview of the Adeno-X Maxi Purification protocol.
ComponentsEquilibration Buffer
Dilution Buffer
Wash Buffer
Elution Buffer
Benzonase® Nuclease (25 U/ml) Syringe-Tip Pre-filter
Adeno-X Maxi Purification Assembly
Syringes (5 and 20 ml) |
Storage Conditions–20°C for Benzonase Nuclease
Room Temperature for all other components |
| Product Name | Size |
Catalog Number |
Price | Add to Cart | Notice to Purchaser |
| Adeno-X Maxi Purification Kit | 2 preps | 631532 | $336.00 USD |
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| Adeno-X Maxi Purification Kit | 6 preps | 631533 | $886.00 USD |
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