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> NucleoSpin® RNA/Protein – Isolation and Purification of RNA & Protein from ONE Lysate
NucleoSpin® RNA/Protein – Isolation and Purification of RNA & Protein from ONE Lysate
- Complete mini kit with rDNase* and shredders
- High-quality RNA suitable for downstream applications
- High protein yield independent of protein size, localization, or modification
- Simplifies transcriptional and translational gene expression profiling
- Easy protein quantification with the Protein Quantification Assay
Applications**
Rapid isolation and purification of total RNA and protein from cultured cells and tissue.
Gene expression profiling, siRNA experiments, analysis of transgenic organisms, and drug screening. Use for RT-PCR, TaqMan® assays, blotting, and microarrays, among others.
Note:
Many different types of starting material have been tested and many proteins have been detected.
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Starting materials successfully used |
Proteins successfully analyzed
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In general: cultured cells / fresh tissue /frozen tissue / tissue in RNAlater® / plants
In detail: cortex of kidney / medulla tissue / liver tissue / neonatal rat cardiomyocytes / macrophages / HeLa / HEK 239 / MCF-7 / D14 SJR / D05 GSM 1A / T47D / 293 / U251 / U373 / HCT116 / GaMG / VH6-TE / garden cress |
In general: cytosolic proteins / membrane proteins / phosphorylated proteins / lipoproteins / glycoproteins / small proteins (17 kDa) / large proteins (250 kDa)
In detail: Cyclin D1 / p53 / Cleaved Casp-3 / Bax / Erk2 / RNA binding protein / ß-Actin / α-Actinin-4 / phospho-ERK1/2 / total-ERK1/2 / γ-Tubulin / MMP1 (active) / pro-MMP / E-Cadherin / prion protein / AMP activated kinase (AMPK) |
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA/Protein system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
** Kits to be used for research purposes only
| Product Name | Size |
Catalog Number |
Price | Add to Cart |
| NucleoSpin RNA/Protein (250 preps) | 250 preps | 740933.250 | $1,330.00 USD |
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| NucleoSpin RNA/Protein (50 preps) | 50 preps | 740933.50 | $293.00 USD |
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| Protein Quantification Assay | 250 preps | 740967.250 | $81.00 USD |
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