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Translational Research | Citation
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Translational Research | Citations

Multiplex PCR

The following are examples of peer-reviewed studies in which Titanium Taq or TaKaRa Taq HS polymerases were used for multiplex PCR in biomedical research:

Polymerase Targets Disease/Biological state studied Reference
Titanium Taq VP2/VP3 region of BK virus Polyomavirus BK 1
Titanium Taq Viral targets VZV, HSV-1, and HSV-2 2
Titanium Taq TMC1 and 8 reference targets Autosomal dominant non-syndromic hearing loss 3
Titanium Taq RAAS genes Hypertension 4
Titanium Taq APP and 11 surrounding genes Alzheimer’s disease 5
TaKaRa Taq HS 8 viruses and subtypes: Influenza A, Influenza A subtypes H1 or H3, Influenza B, RSV, hMPV, Rhinovirus, and Adenovirus Viral respiratory diseases 6
TaKaRa Taq HS Rary Fyb and S alleles Genotyping rare blood types 7
TaKaRa Taq HS HPV type 16/18/33 DNA Human papillomavirus (HPV) 8
TaKaRa Taq HS and Titanium Taq NAT2 genotyping Drug metabolism 9

Titanium Taq DNA Polymerase

  1. Stellrecht, K. A., et al. (2013) Comparison of three real-time PCR for the quantification of polyomavirus BK. J. Clin. Virol. 56:354–359.

    Summary: Latent polyomavirus BK, if reactivated during immunosuppression, can cause complications for kidney transplant patients. Researchers compared three different multiplex real-time PCR assays to analyze samples from 69 renal transplant patients for BK viral load. Titanium Taq polymerase was used with BK Virus Primers ASR from Luminex (MC-RTx assay) to amplify BK virus sequences from the VP2/VP3 region. The MC-RTx assay using Titanium Taq was found to have the greatest sensitivity and highest performance characteristics among the assays tested.

  2. Buelow, D. R., et al. (2013) Comparison of two multiplexed PCR assays for the detection of HSV-1, HSV-2, and VZV with extracted and unextracted cutaneous and mucosal specimens. J. Clin. Virol. 58:84–88.

    Summary: Varicella zoster virus (VZV) and Herpes Simplex Virus 1 and 2 (HSV-1, HSV-2) can cause life-threatening infections in immunocompromised individuals. The authors examined the performance of analyte specific reagents (ASR) that were multiplexed for simultaneous detection of VZV, HSV-1, and HSV-2 from cutaneous or mucosal lesion clinical specimens. Titanium Taq was used for multiplex real-time PCR with Luminex (EraGen) ASR primer sets (MultiCode HSV primers, MultiCode VZV primers, and control primers) and was compared with another multiplexed commercial ASR assay (Focus Diagnostics). Successful multiplex analysis of dermal specimens was reported for both ASR assays.

  3. Hilgert, N., et al. (2008) Mutation analysis of TMC1 identifies four new mutations and suggests an additional deafness gene at loci DFNA36 and DFNB7/11. Clin. Genetics 74:223–232.

    Summary: To understand the genetic heterogeneity of deafness, researchers analyzed 51 families with a history of autosomal dominant non-syndromic hearing loss. The samples were screened for copy number variation in the known disease-causing gene Transmembrane channel-like gene 1 (TMC1) using multiplex amplicon quantification (MAQ) with Titanium Taq polymerase. Twelve test amplicons located in the TMC1 locus and eight reference amplicons were successfully amplified simultaneously.

  4. Jiang, X., et al. (2007) Effect of renin-angiotensin-aldosterone system gene polymorphisms on blood pressure response to antihypertensive treatment. Chin. Med. J. 120(9):782–786.

    Summary: Researchers identified polymorphisms in genes in the renin-angiotensin-aldosterone system (RAAS) among a population of Chinese Han patients with essential hypertension treated with an antihypertensive agent. Titanium Taq polymerase was used in a multiplex PCR genotyping assay covering seven amplicons. Data were correlated with blood pressure response to therapy to reveal various genotypes that correlated with positive therapeutic response.

  5. Brouwers, N., et al. (2006) Genetic risk and transcriptional variability of amyloid precursor protein in Alzheimer’s disease. Brain 129:2984–2991.

    Summary: This genetic variation study analyzed the incidence of genomic locus duplication and presence of mutations in the promoter region of the amyloid precursor protein (APP) gene. Titanium Taq polymerase was used for multiplex amplicon quantification (MAQ) PCR assays to assess the copy number of APP and 11 neighboring genes. A total of 37 different amplicons were amplified in each multiplex PCR assay.

TaKaRa Taq HS DNA Polymerase

  1. Landes, M. B., et al. (2013) The frequency and seasonality of influenza and other respiratory viruses in Tennessee: two influenza seasons of surveillance data, 2010–2012. Influenza and Other Resp. Viruses 7(6):1122–1127.

    Summary: Nasal and nasopharangeal swabs/washings from 2247 individuals with influenza-like symptoms were collected across Tennessee. Influenza and other respiratory viruses were identified using a molecular respiratory virus panel, the xTAG Respiratory Viral Panel (RVP), on the Luminex xMAP platform. TaKaRa Taq HS polymerase was used as a Qualified Ancillary Reagent in this protocol. The xTAG RSV assay covers 8 different viruses and viral subtypes (Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza B, RSV, hMPV, Rhinovirus, and Adenovirus).

  2. He, Y.-L., et al. (2012) Multiplex polymerase chain reaction with DNA pooling: a cost-effective strategy of genotyping rare blood types. Transfusion Medicine 45:937–941.

    Summary: Researchers at the Shanghai Blood Center and East China Normal University reported a multiplex PCR screening method for detection of rare Fyb and S alleles using TaKaRa Taq HS DNA Polymerase. A total of 4,490 donor samples were screened in 898 pools (each pool n=5). Positive pools were then subjected to further testing.

  3. Iwakawa, R., et al. (2010) Prevalence of human papillomavirus 16/18/33 infection and p53 mutation in lung adenocarcinoma. Cancer Sci. 101(8):1891–1896.

    Summary: To elucidate whether HPV types 16, 18, and 33 are involved in the development of lung adenocarcinoma in Japan, the authors screened for viral DNA in 275 primary and 22 metastatic lung adenocarcinoma samples from Japanese patients and 91 different lung cancer cell lines. The assay involved multiplex PCR with type-specific primers and TaKaRa Taq HS polymerase.

  4. Zhu, Y., et al. (2006) Simultaneous determination of 7 N-Acetyltransferase-2 single-nucleotide variations by allele-specific primer extension assay. Clinical Chem. 52(6):1033–1039.

    Summary: Researchers at the Louisville School of Medicine used TaKaRa Taq HS in comparison to Platinum GenoType Tsp (Invitrogen) or Titanium Taq (Clontech) for a multiplex allele-specific primer extension (ASPE) assay to genotype NAT2, which encodes a protein involved in activation/deactivation of aromatic amine drugs and carcinogens. All three polymerases tested were found to perform acceptably in the ASPE assay.

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