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Translational Research | Genomics
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Translational Research | Genomics

Behind every good targeted resequencing experiment, there’s a great polymerase


Because targeted resequencing using Next Generation Sequencing (NGS) platforms allows detailed investigation of specific genomic regions, this technique is frequently used to assess somatic or germline mutations. Targeted resequencing also aids discovery of novel causative mutations for genetic disorders. DNA samples are amplified by an initial round of PCR, then pooled for library preparation and NGS. This first amplification step is critical to success. For targeted resequencing workflows with a variety of NGS platforms we offer PrimeSTAR GXL DNA Polymerase and TaKaRa LA Taq HS DNA Polymerase.

Applications Product length Fidelity High %GC template
PrimeSTAR GXL
  • Citation list >>
  • Point mutations
  • GC-rich, AT-rich, or repetitive sequences
  • Rare alleles
  • Limiting sample amounts
  • ≤ 30 kb (genomic)
  • ≤ 13.5 kb (cDNA)
High
(1 error/16,230 bp tested on GC-richThermus thermophilus HB8 genomic DNA)
Recommended
TaKaRa LA Taq HS
  • Citation list >>
  • Long-range PCR
  • Breakpoint regions
  • T cell receptor loci rearrangements
  • mtDNA mutations
  • Genome variation
  • ≤ 30 kb (genomic)
Moderate
(approx.6.5X higher fidelity than Taq)
 

Contact us for Royalty-free, Glycerol-Free, OEM or Custom Products

Interested in royalty-free DNA polymerase formulations, OEM, glycerol-free enzymes, custom packaging or product configurations? Please inquire at licensing@clontech.com or submit an inquiry online.

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