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Thats_Good_Science >  Translational_Research >  Products >  Sample_Prep >  Selection_Guide_Kits_for_RNA_Purification

RNA Purification Guide
RNA Purification Guide
  Starting material Yield Format Auto. Vs Manual? You Need:
Cells & tissue (total RNA)
  • <30 mg tissue
  • <5 x 106 cultured cells
14–70 µg Mini spin columns M NucleoSpin RNA
(available for fully automated sample prep using QiaCube)
  • 1–100,000 cultured cells
  • <5 mg tissue
0.1–1,500 ng XS spin columns M NucleoSpin RNA XS
  • <5 x 107 cultured cells
  • 200 mg tissue
  • 180 µg from 107 HeLa cells
  • 620 µg from 4 x 107 HeLa cells
Midi spin columns M NucleoSpin RNA Midi
  • <107 cultured cells
  • 30 mg tissue
<100 µg 8-well strips M NucleoSpin 8 RNA
  • <107 cultured cells
  • 30 mg tissue
<100 µg 96-well plates M/A NucleoSpin 96 RNA
  • 20 mg tissue
  • 2 x 106 cells
<30 µg Magnetic M/A NucleoMag 96 RNA
FFPE samples (total RNA)
  • 4–10 sections (10 µm) of up to 6 mm
  • 2 total area
  • <50 mg tissue
Depends on amount & 
quality of the sample
Mini spin columns M NucleoSpin total RNA FFPE
  • 1–3 sections (10 µm) of up to 6 mm
  • 2 total area
  • <5 mg tissue
Depends on amount & 
quality of the sample
XS spin column M/A NucleoSpin total RNA FFPE XS
Blood (total RNA) <400 µl whole blood 
(fresh or frozen)
1–8 µg (400 µl whole blood) depending on leukocyte number Mini spin columns M NucleoSpin RNA Blood
400–1,300 µl whole blood 4–26 µg (1,300 µl whole blood) depending on leukocyte number Midi spin columns M NucleoSpin RNA Blood Midi
miRNA
  • <107 cultured cells
  • <30 mg tissue
10 µg small RNA, 95 µg large RNA from 107 HeLa cells Mini spin columns M NucleoSpin miRNA
300 µl plasma or serum (up to 900 µl with multiple loadings)   Mini spin columns M NucleoSpin miRNA Plasma
mRNA 250 µg total RNA 10 µg mRNA Oligo(dT) latex bead susp. M NucleoTrap mRNA Mini
1,000 µg total RNA 40 µg mRNA Oligo(dT) latex bead susp. M NucleoTrap mRNA Midi
Viral RNA from clinical samples 150 µl–1 ml serum, plasma, or cell-free biological fluids Varies by sample Mini spin columns M NucleoSpin RNA Virus
150 µl (100 cp/ml) serum, plasma, or cell-free biological fluids Varies by sample 8-well strips M/A NucleoSpin 8 Virus
150 µl (100 cp/ml) serum, plasma, or cell-free biological fluids) Varies by sample 96-well plates M/A NucleoSpin 96 Virus
200 µl serum or plasma Varies by sample Magnetic M/A NucleoMag 96 Virus
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