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Thats_Good_Science >  Translational_Research >  Products >  Sample_Prep >  Selection_Guide_Kits_for_Genomic_DNA_Purification

Genomic DNA purification
Genomic DNA purification
  Starting material Yield Format Auto. Vs Manual? You Need:
Tissue

1–25 mg tissue

102–107 cultured cells

20–35 µg Mini spin columns M/A NucleoSpin Tissue
(available for fully automated sample prep using QiaCube)

0.025–10 mg tissue

10–10,000 cultured cells

10,000 HeLa cells: 10–50 ng XS spin columns M NucleoSpin Tissue XS
<20 mg tissue 15–25 µg 8-well strips or 96-well plates M/A NucleoSpin 8 Tissue or NucleoSpin 96 Tissue
<20 mg tissue 10–20 µg Magnetic beads M/A NucleoMag 96 Tissue

<20 mg tissue (mini)

<100 mg tissue (midi)

<400 mg tissue (maxi)

20 µg (mini)

100 µg (midi)

500 µg (maxi)

anion-exchange columns M NucleoBond AXG 20 (mini), 100 (midi), or 500 (maxi)
FFPE samples

<7 sections (10 µm) of 250 mm2 total area

<15 mg paraffin (standard protocol; use modified protocol for larger amounts)

Depends on amount & quality of the sample Mini spin columns – XS designa M NucleoSpin FFPE DNA
Blood*

1–200 µl whole blood (mini)

0.2–2 ml whole blood (midi)

2–10 ml whole blood (maxi)

4–6 µg (mini)

40–60 µg (midi)

200–300 µg (maxi)

Mini, midi, or maxi silica membrane spin columns M NucleoSpin Blood (mini), NucleoSpin Blood L (midi), or NucleoSpin Blood XL (maxi)
1–200 µl whole blood 4–6 µg Fast mini spin columns M NucleoSpin Blood QuickPure

2–5 ml whole blood (midi)

5–20 ml whole blood (maxi)

100 µg (midi)

500 µg (maxi)

anion-exchange columns M NucleoBond CB 100 (midi) or 500 (maxi)
1–200 µl whole blood 4–6 µg 8-well strips or 96-well plates M/A NucleoSpin 8 Blood or NucleoSpin 96 Blood
200 µl whole blood 4–6 µg Fast 8-well strips or 96-well plates M NucleoSpin 8 or 96 Blood QuickPure
200 µl whole blood 2–8 µg Magnetic beads M/A NucleoMag Blood
Plasma 240 µl plasma or serum 0.1–100 ng per ml plasma, depending on sample XS spin columns M NucleoSpin Plasma XS
Forensic samples Forensic samples, buccal swabs, blood spots >10 ng Funnel columns M NucleoSpin DNA Trace

Forensic samples, buccal swabs, blood spots, cigarette filters (NucleoSpin 8 trace)

Forensic samples, buccal swabs, blood spots (NucleoSpin 96 Trace)

1–2 µg 8-well strips or 96-well plates M/A NucleoSpin 8 or 96 Trace
Viral DNA from clinical samples

150 µl (NucleoSpin RNA Virus)

1 ml (NucleoSpin RNA Virus F)

Varies by sample. Simultaneous isolation of viral RNA and DNA (supporting protocol). Mini spin columns (regular) or funnel columns (F) M NucleoSpin RNA Virus or NucleoSpin RNA Virus F

1–200 µl whole blood (mini)

0.2–2 ml whole blood (midi)

2–10 ml whole blood (maxi)

Varies by sample. Recommended for isolation of viral DNA from blood samples with the highest sensitivity. Mini, midi, or maxi silica membrane spin columns M NucleoSpin Blood (mini), NucleoSpin Blood L (midi), or NucleoSpin Blood XL (maxi)
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