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A simple way to optimize concentrations of vector and insert for your In-Fusion cloning reactions. All you need to do is enter your vector and insert sizes in base pairs.
Use this tool to add In-Fusion adaptors (vector-specific sequences approximately 15 bp long) to the 5'-end of your primers.
Enter a cDNA sequence or an accession number (RefSeq and GenBank are accepted) for your gene of interest to generate a list of candidate sequences that can be targeted by RNAi.
Enter the sense sequence that you want the full pSIREN compatible sequence designed for.