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A Complete Workflow for Generating Recombinant Adeno-Associated Viral Vectors

Recombinant adeno-associated virus (AAV) vectors are popular tools for gene delivery to mammalian cells and for gene therapy. Clontech now offers a complete workflow for AAV particle production:

  • AAV Packaging—Unique system generates high titers of AAV particles without the use of a helper virus.
  • AAV Particle Extraction—Extraction solution increases particle recovery by ≥3-fold as compared to conventional freeze-thaw methods.
  • AAV Particle Purification—Column-based method for obtaining high purity, high titer AAV particles suitable for in vivo transduction.
  • AAV Titration—Precise quantification using qPCR.

Simplify genome editing with efficient screening of insertions and deletions

The Guide-it Mutation Detection Kit contains all the reagents you need to quickly and easily identify and confirm insertions or deletions generated using genome editing tools such as ZFNs, TALENs, or CRISPR/Cas9. Just amplify your putative target sequence directly from cells, screen the amplicon for your desired edits using a simple hybridization and enzymatic cleavage procedure, and analyze the cleavage products on an agarose gel to determine cleavage efficiency.

  • Complete kit—includes PCR reagents, a cleavage enzyme, and 4X as many reactions as competitor kits
  • Identify a target sequence directly from cells, without genomic DNA extraction
  • Easily measure target cleavage efficiency using agarose gel analysis

Highly efficient transfection in a convenient dry-down format

The superior transfection efficiency and low cytotoxicity of the Xfect Transfection Reagent is now available in Xfect Single Shots (Midi)—lyophilized, single-transfection aliquots of the Xfect reagent supplied in individual tubes. The amount of reagent in each midi-format tube is optimized for a single well of a 6-well plate. Transfections can be carried out entirely in the presence of serum.

  • High transfection efficiency and very low cytotoxicity
  • Transfect a broad range of mammalian cell types
  • Simple serum-compatible protocol

Inducible expression that’s as easy as constitutive expression

Until now the best inducible mammalian expression systems have all required a two-vector format. The Tet-One Inducible Expression Systems are the only commercially available single-vector systems that can deliver a 3–4 orders of magnitude difference in expression levels between induced and uninduced states. These systems are available in lentiviral, retroviral, and plasmid-based formats.

  • No need to create double-stable cell lines
  • Obtain >10,000-fold induction
  • Viral systems include a high-titer packaging system

Strand-specific RNA-seq libraries for Illumina platforms

The SMARTer Stranded RNA-Seq Kit generates indexed libraries that retain strand-specific information suitable for Illumina sequencing. This all-inclusive kit requires a simple 3.5 hour protocol and provides unparalleled sensitivity.

  • Works with as little as 100 pg of RNA
  • Creates multiplexed libraries in less than 4 hours
  • Includes SeqAmp DNA Polymerase, a new PCR enzyme that performs well even with challenging templates
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