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Reliable production of high-titer lentivirus in a single step

Lenti-X Packaging Single Shots (VSV-G) provide an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because this product consists of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells in a 10-cm dish.

  • Exceptionally high titers (107–108 IFU per ml)
  • Simple protocol—Just add your lentiviral DNA, mix, and add to 293T cells
  • No optimization or additional transfection reagent required

ChIP-Seq Libraries Powered by DNA SMART Technology

Chromatin immunoprecipitation (ChIP) experiments often yield very small amounts of DNA. Unfortunately, most next-generation sequencing (NGS) library preparation methods use ligation to add sequencing adapters and require a larger amount of DNA. The DNA SMART ChIP-Seq Kit eliminates this problem; instead of using ligation, Illumina® sequencing adapters are added directly to low-input ChIP DNA samples via a novel, SMART-based template switching technology. Eliminating ligation from library preparation means only a single, combined size selection and clean-up step is needed, leading to higher yield and a simpler workflow.

  • Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA, or total DNA from 10,000–1,000,000 cell ChIP experiments
  • Illumina-ready sequencing libraries in around 4 hours: Illumina indexes and adaptors incorporated into the workflow
  • Excellent sequencing metrics: High non-redundant rate, high reproducibility between different sample inputs, and high overlap with data generated by the ENCODE project

Learn about all of our products for NGS: www.clontech.com/NGS

Analysis of Indels Introduced by CRISPR/Cas9 Gene Editing in Four Steps

PCR, clone, PCR, sequence―with the Guide-it Indel Identification Kit, characterizing mutations introduced by genome editing is as simple as that. The kit includes all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis. Plus, with the ultra-streamlined protocol that includes time-saving Terra PCR Direct Polymerase and the innovative In-Fusion cloning system, reliable sequencing results can be obtained in a matter of days.

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