login | register | order now | view cart | feedback

Support >  New_Products

New Products

A New Kit for Analysis of Human T-Cell Receptor mRNA Sequences

NGS technology is increasingly being applied to the study of human T-cell receptor (TCR) repertoire diversity. However, common approaches for generating TCR sequencing libraries typically include only portions of TCR variable regions, and often involve complicated multiplex PCR strategies. The SMARTer Human TCR a/b Profiling Kit employs a 5' RACE-like approach to capture complete V(D)J variable regions of TCR-α and/or TCR-β subunits, utilizing a simple, semi-nested PCR strategy for library construction. Libraries produced with the kit are indexed and ready for sequencing on Illumina® platforms. The SMARTer approach:

  • Yields full-length sequence information for variable regions of TCR-α and/or TCR-β subunits
  • Avoids multiplex PCR and minimizes the likelihood of amplification biases during library production
  • Generates high-quality sequencing libraries from 10 ng–3 μg of total RNA or 50–10,000 purified T cells
  • Allows for detection of low-abundance TCR clonotypes, even at shallow sequencing depths

High-Yield, High-Purity His-Tagged Protein Preps in Minutes

Capturem his-tagged protein purification kits let you take advantage of next-generation membrane technology and leave the cold room behind. Now you can isolate and concentrate diluted protein in one step, and use the additives you need—most are compatible with Capturem technology. This unique system, which provides high-speed, high-capacity protein purification right at your bench, is available in miniprep, maxiprep, and 96-well plate formats.

GMP-Grade Polymerase for Quality Assurance in Demanding Research Applications

Our deep enzymology expertise is now paired with GMP compliance, bringing an added level of confidence and consistency to the robust, sensitive PCR enzymes researchers have come to rely on. We are proud to offer a new version of one of our most popular hot-start enzymes, ideal for demanding assays like multiplex PCR. TaKaRa Taq Hot Start Version GMP grade boasts high specificity and excels with viral samples and qPCR. Additionally, this new GMP-compliant product offers:

  • Stringent specifications
  • High performance
  • Custom sizes and packaging

High-Sensitivity RNA-Seq Libraries from Single Cells Captured with the Fluidigm C1 System

The SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System allows for synthesis of high-quality cDNA from IFC-captured cells (96-cell IFC format) processed with the Fluidigm C1 Single-Cell Auto Prep System. Employing SMART technology and primers enhanced with locked nucleic acids (LNAs), this kit offers remarkable sensitivity, enriches for full-length transcripts, and maintains true representation of sample mRNA—critical factors for transcriptome analysis. This kit:

  • Provides increased sensitivity relative to the previous generation (the SMARTer Ultra Low Input RNA Kit for the Fluidigm C1 System, IFC), detecting more genes
  • Yields more accurate expression data for genes with high GC content
  • Generates cDNA libraries tested for compatibility with Illumina® sequencing platforms

Fast, High-Capacity, Small-Scale Antibody Purification and Screening

Capturem Protein A Miniprep columns provide rapid, high-quality, small-scale purification and screening of monoclonal and polyclonal antibodies. These miniprep columns combine the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification. These columns are convenient, powerful, and efficient tools for small-scale antibody purification, and offer the following advantages:

  • Fast and easy protocol allows for purification of antibodies in 5 minutes at room temperature
  • Short residence times ensure antibody quality by reducing the possibility of aggregation
  • Small column bed volumes trap fewer contaminants and allow elution of concentrated antibodies
  • Capturem membrane technology eliminates the need for media exchange

Efficient CRISPR/Cas9-Mediated Gene Editing with No Additional Footprint

While CRISPR/Cas9 is a powerful technique for gene manipulation, plasmid delivery of Cas9 and sgRNA can be difficult with certain cell types, and the possibility of an additional footprint (i.e., persistent and elevated expression of Cas9) is present with both plasmid and viral delivery. To address these challenges, we developed the Guide-it CRISPR/Cas9 Gesicle Production System. Gesicles are cell-derived nanovesicles containing active Cas9 protein complexed with your specific sgRNA. This complete kit contains everything you need to easily produce your own gesicles and take full control of your targeted gene editing experiments:

  • Efficient delivery to a broad range of cell types
  • Direct delivery of Cas9 protein, as opposed to DNA, eliminates genomic integration and reduces off-target effects
  • Tight control over dose and timing of Cas9 delivery and gene editing

Functional Hepatocytes for Toxicity, Metabolism, and Drug Development Studies

Get the ready-made cells you need with hepatocytes derived from human induced pluripotent stem (hiPS) cells, or use the Cellartis iPS Cell to Hepatocyte Differentiation System to turn your disease- or patient-specific hiPS cells into customized hepatocytes. Hepatocytes obtained with this system express major hepatic markers, show relevant CYP enzyme activities, and are functionally stable for a longer timeframe compared to cryopreserved primary hepatocytes, enabling prolonged experiments such as long-term toxicity assays or viral infection studies. The hepatocyte differentiation system:

  • Consistently generates >90% pure hepatocytes, ideal for drug metabolism and safety studies
  • Uses a protocol that has been validated on over 25 different iPS cell lines, so no need to optimize
  • Contains the Cellartis Definitive Endoderm Differentiation Kit (for producing DE cells from iPS cells) and the Cellartis Hepatocyte Differentiation Kit (for producing hepatocytes from DE cells)
Clontech is a Takara Bio Company © 2016 Privacy Policy | Terms & Conditions