Protein-protein interactions trigger signal transduction cascades, induce protein accumulation at specific cellular locations, and control a host of other biological processes. One challenge in artificial control of protein-protein interactions is that proteins can dimerize simply because they are in close physical proximity—particularly if they are membrane proteins.
Now you can eliminate these unwanted events: we’ve combined iDimerize and Tet-On 3G technologies in the iDimerize Inducible Homodimer and Heterodimer Systems (with Tet-On 3G) to give you two-tiered control of protein behaviors:
- First, use doxycycline to optimize the proteins’ expression to physiologically-relevant levels
- Then induce dimerization by adding the dimerizer ligand to your culture medium