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New Products

End-capture RNA-Seq for High-Throughput Differential Expression Analysis

The SMART-Seq v4 3’ DE Kit is designed to preferentially amplify the 3’ ends of RNA transcripts. This kit also incorporates cell barcodes in the form of in-line indexes in order to enable pooling of up to 12 samples per paired Illumina index or up to 1,152 samples per sequencing lane. This kit utilizes the high sensitivity of the SMART-Seq v4 technology while improving throughput and reducing the number of reads required for gene identification. 

  • Focus sequencing on the 3’ ends of transcripts
  • Run up to 1,152 samples per sequencing lane
  • Simplify single cell differential expression analysis
  • Reduce experimental cost of differential expression studies

This is an excellent kit for differential expression analysis on a large number of samples.

Targeted RNA-Seq for Full-Length Transcript and Gene Fusion Analysis

SMARTer Target RNA Capture for Illumina is a complete system designed to directly capture targets of interest from total RNA. This new method for targeted RNA-seq is highly sensitive, provides full-length sequence information and improved sequence coverage, and reduces experimental costs. In the streamlined protocol, hybridization is completed in under four hours, followed by capture of RNA-DNA complexes using Capture Beads and then cDNA synthesis.

  • Directly capture information from RNA
  • Generate high-quality data from just 10 ng of total RNA
  • Choose the ideal tool for gene fusion analysis
  • Prepare Illumina-ready cDNA libraries in a ligation-free manner
  • Identify chimeric gene fusions, transcript isoforms, and splice variants

New Xeno-Free and GMP-Grade Stem Cell Media Products to Accelerate Discovery

Moving human pluripotent cell-based therapies from the lab to the clinic involves jumping over key hurdles in the process, and our Cellartis brand xeno-free media products are designed with these challenges in mind. Stemming from more than 15 years of expertise in pluripotent cell generation and differentiation, our new additions to the xeno-free media product family aim to maximize the potential of your cell therapy and ultimately influence human health.

  • Our original xeno-free medium promotes robust 2D monolayer expansion and is suitable for preclinical and research applications.
  • Our NEW xeno-free 3D spheroid culture medium is optimized for scale-up of iPSCs as 3D spheroids for downstream directed differentiation—5 x 109 cells in a 1 liter bioreactor within 3–4 passages.
  • Our NEW xeno-free GMP-grade basal medium is manufactured with clinical-quality materials and traceable production processes, and is ideal for small- or large-scale production of pluripotent cells for downstream use in clinical research settings.

Efficient T-Cell Culture and Expansion Medium

Promoting robust T-cell expansion is an important prerequisite for basic and preclinical studies, including adoptive cell therapy studies. GT-T551 Culture medium, 1L Bottle is ideally suited for expansion of T cells, and is recommended in our protocol for anti-CD3/IL-2 T-cell activation in conjunction with RetroNectin. This protocol results in an expanded cell population that shows a higher proportion of naïve T cells than anti-CD3 antibody stimulation alone. This T cell medium is:

  • Supplemented with human serum albumin, human insulin, L-glutamine, and streptomycin
  • Endotoxin tested (endotoxin <0.3 EU/ml)
  • Compatible with CultiLife Culture Bags for large-scale expansion of T cells

A New Kit for Small RNA-Seq

We are excited to announce the development of the SMARTer smRNA-Seq Kit for Illumina, designed specifically for next-gen sequencing of small RNAs. This kit incorporates features from our industry-leading SMART-Seq v4 kit, including SMART technology and locked nucleic acids, to provide the highest sensitivity available. Libraries are generated in a ligation-free manner, ensuring that diverse small RNAs are represented with minimal bias. Features include:

  • Prepare sequencing libraries for small RNAs 15–150 nt in length, including miRNAs, siRNAs, piRNAs, and snoRNAs
  • Start from 1 ng–2 µg of total RNA or enriched small RNA
  • Incorporate Illumina adapters and indexes in a ligation-free manner for sample multiplexing
  • Generate sequencing libraries in ~3 hours and enrich for small RNAs of interest using a gel-free protocol

Efficient CRISPR/Cas9-Mediated Gene Editing with No Additional Footprint

While CRISPR/Cas9 is a powerful technique for gene manipulation, plasmid delivery of Cas9 and sgRNA can be difficult with certain cell types, and the possibility of an additional footprint (i.e., persistent and elevated expression of Cas9) is present with both plasmid and viral delivery. To address these challenges, we developed the Guide-it CRISPR/Cas9 Gesicle Production System. Gesicles are cell-derived nanovesicles containing active Cas9 protein complexed with your specific sgRNA. This complete kit contains everything you need to easily produce your own gesicles and take full control of your targeted gene editing experiments:

  • Efficient delivery to a broad range of cell types
  • Direct delivery of Cas9 protein, as opposed to DNA, eliminates genomic integration and reduces off-target effects
  • Tight control over dose and timing of Cas9 delivery and gene editing

Functional Hepatocytes for Toxicity, Metabolism, and Drug Development Studies

Get the ready-made cells you need with hepatocytes derived from human induced pluripotent stem (hiPS) cells, or use the Cellartis iPS Cell to Hepatocyte Differentiation System to turn your disease- or patient-specific hiPS cells into customized hepatocytes. Hepatocytes obtained with this system express major hepatic markers, show relevant CYP enzyme activities, and are functionally stable for a longer timeframe compared to cryopreserved primary hepatocytes, enabling prolonged experiments such as long-term toxicity assays or viral infection studies. The hepatocyte differentiation system:

  • Consistently generates >90% pure hepatocytes, ideal for drug metabolism and safety studies
  • Uses a protocol that has been validated on over 25 different iPS cell lines, so no need to optimize
  • Contains the Cellartis Definitive Endoderm Differentiation Kit (for producing DE cells from iPS cells) and the Cellartis Hepatocyte Differentiation Kit (for producing hepatocytes from DE cells)

High-Sensitivity RNA-Seq Libraries from Single Cells Captured with the Fluidigm C1 System

The SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System allows for synthesis of high-quality cDNA from IFC-captured cells (96-cell IFC format) processed with the Fluidigm C1 Single-Cell Auto Prep System. Employing SMART technology and primers enhanced with locked nucleic acids (LNAs), this kit offers remarkable sensitivity, enriches for full-length transcripts, and maintains true representation of sample mRNA—critical factors for transcriptome analysis. This kit:

  • Provides increased sensitivity relative to the previous generation (the SMARTer Ultra Low Input RNA Kit for the Fluidigm C1 System, IFC), detecting more genes
  • Yields more accurate expression data for genes with high GC content
  • Generates cDNA libraries tested for compatibility with Illumina® sequencing platforms

A New Kit for Analysis of Human T-Cell Receptor mRNA Sequences

NGS technology is increasingly being applied to the study of human T-cell receptor (TCR) repertoire diversity. However, common approaches for generating TCR sequencing libraries typically include only portions of TCR variable regions, and often involve complicated multiplex PCR strategies. The SMARTer Human TCR a/b Profiling Kit employs a 5' RACE-like approach to capture complete V(D)J variable regions of TCR-α and/or TCR-β subunits, utilizing a simple, semi-nested PCR strategy for library construction. Libraries produced with the kit are indexed and ready for sequencing on Illumina® platforms. The SMARTer approach:

  • Yields full-length sequence information for variable regions of TCR-α and/or TCR-β subunits
  • Avoids multiplex PCR and minimizes the likelihood of amplification biases during library production
  • Generates high-quality sequencing libraries from 10 ng–3 μg of total RNA or 50–10,000 purified T cells
  • Allows for detection of low-abundance TCR clonotypes, even at shallow sequencing depths

High-Yield, High-Purity His-Tagged Protein Preps in Minutes

Capturem his-tagged protein purification kits let you take advantage of next-generation membrane technology and leave the cold room behind. Now you can isolate and concentrate diluted protein in one step, and use the additives you need—most are compatible with Capturem technology. This unique system, which provides high-speed, high-capacity protein purification right at your bench, is available in miniprep, maxiprep, and 96-well plate formats.

GMP-Grade Polymerase for Quality Assurance in Demanding Research Applications

Our deep enzymology expertise is now paired with GMP compliance, bringing an added level of confidence and consistency to the robust, sensitive PCR enzymes researchers have come to rely on. We are proud to offer a new version of one of our most popular hot-start enzymes, ideal for demanding assays like multiplex PCR. TaKaRa Taq Hot Start Version GMP grade boasts high specificity and excels with viral samples and qPCR. Additionally, this new GMP-compliant product offers:

  • Stringent specifications
  • High performance
  • Custom sizes and packaging

Fast, High-Capacity, Small-Scale Antibody Purification and Screening

Capturem Protein A Miniprep columns provide rapid, high-quality, small-scale purification and screening of monoclonal and polyclonal antibodies. These miniprep columns combine the specific antibody-binding properties of Protein A with novel Capturem technology to provide high-capacity, membrane-based affinity purification. These columns are convenient, powerful, and efficient tools for small-scale antibody purification, and offer the following advantages:

  • Fast and easy protocol allows for purification of antibodies in 5 minutes at room temperature
  • Short residence times ensure antibody quality by reducing the possibility of aggregation
  • Small column bed volumes trap fewer contaminants and allow elution of concentrated antibodies
  • Capturem membrane technology eliminates the need for media exchange
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