Obtaining consistent, reproducible results with lentiviral transduction requires applying similar amounts of virus in each experiment, but many labs avoid titering their lentiviral preps because conventional methods such as ELISA, qPCR, or functional assays are cumbersome and time consuming. Lenti-X GoStix Plus alleviate the burdens associated with lentivirus titration by enabling rapid, reliable quantitation in 10 minutes with easy-to-use cassettes and a smartphone app.
Titering your lentiviral preps is easy: simply apply 20 µl of supernatant to a GoStix cassette, wait 10 minutes for the appearance of test and control bands, then scan the bands with our free smartphone app. The quantitative output from the Lenti-X GoStix App (referred to as a GoStix Value, or GV) can be used to compare virus amounts between different preparations in a manner similar to, but far easier and quicker than, an ELISA and allows for calculation of actual IFU/ml using a reference virus.
- Obtain quantitative lentiviral titers in 10 minutes using a simple smartphone app
- Compare virus amounts between different preparations
- Determine the optimal time to harvest lentiviral supernatants
- Achieve consistent performance for your experiments