The Guide-it Mutation Detection Kit contains all the reagents you need to quickly and easily identify and confirm insertions or deletions generated using genome editing tools such as ZFNs, TALENs, or CRISPR/Cas9. Just amplify your putative target sequence directly from cells, screen the amplicon for your desired edits using a simple hybridization and enzymatic cleavage procedure, and analyze the cleavage products on an agarose gel to determine cleavage efficiency.
- Complete kit—includes PCR reagents, a cleavage enzyme, and 4X as many reactions as competitor kits
- Identify a target sequence directly from cells, without genomic DNA extraction
- Easily measure target cleavage efficiency using agarose gel analysis