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Rapid his-tagged protein purification from large lysate volumes

Capturem his-tagged protein purification kits let you take advantage of next-generation membrane technology and leave the cold room behind. Now you can isolate and concentrate diluted protein in one step, and use the additives you need—most are compatible with Capturem technology. This unique system, which provides high-speed, high-capacity protein purification right at your bench, is now available in Capturem His-Tagged Purification Large Volume, single-use, disposable bottle-top units for use with up to 500 ml of cleared cell lysate, in addition to our miniprep, maxiprep, and 96-well plate formats. The new purification units are:

  • Fast and convenient: work for 15–30 min at room temperature instead of 3–4 hr in the cold room
  • High-purity and high-yield: elute up to 25 mg/unit at a concentration of up to 2.0 mg/ml (depending on protein size and expression level)
  • High-capacity: columns contain novel nylon membranes instead of resin
  • Flexible: purify his-tagged proteins from mammalian or bacterial cells

High-quality, high-yield exosome isolation in minutes

Combining Capturem technology with a proprietary nonantibody-based exosome binding compound, the Capturem Exosome Isolation Kit (Cell Culture) provides a simple and rapid solution for the isolation and enrichment of exosomes from cell culture medium. The Capturem-isolated exosomes display the physical, biochemical, and biological properties, considered to be common exosome characteristics. Compared to ultracentrifugation- and precipitation-based methods, the Capturem kit produces higher yields of exosomes, allowing researchers to perform more downstream analyses with each prep.

  • Isolates exosomes in under 30 minutes without ultracentrifugation—from cell culture supernatant collection through elution
  • Provides all the necessary columns and buffers—preclearing columns to remove cellular debris, isolation columns for exosome binding, and optimized buffers for the isolation
  • Yields up to 2 x 1011 of purified exosomes per column, depending on cell type and cell culture conditions

Efficient system for generating long ssDNA donor fragments for knockin CRISPR experiments

We are pleased to announce the release of the Guide-it Long ssDNA Production System, designed to produce a long single-stranded DNA (ssDNA) oligo of up to 5 kb in length, for use as a repair template in knockin experiments using CRISPR/Cas9 or other genome editing tools. One of the major challenges of knockin experiments is the production and delivery of the repair template along with the Cas9-sgRNA ribonucleoprotein complex. Although ssDNA repair templates have been shown to be more effective for gene editing than double-stranded DNA, their use is limited due to the difficulty of producing long templates or needing to use adeno-associated virus (AAV) for delivery. The Guide-it Long ssDNA Production System provides a fast and simple in vitro method for generating long ssDNA donor templates, which do not randomly integrate into the genome, are less toxic to cells, and do not require AAV production, resulting in improved gene editing efficiency. 

  • ssDNA does not randomly integrate into the genome, unlike dsDNA
  • ssDNA does not express unless properly integrated into the genome, making identification of correctly edited cells more accurate when delivering expression cassettes
  • ssDNA causes significantly less cytotoxicity in edited cells when compared with dsDNA
  • Create highly pluripotent, edited clonal hiPSC lines with optimized Cellartis editing and culture systems

    Looking for a complete system to create CRISPR/Cas9-edited human iPS cell lines? Our carefully designed, “start-to-finish” editing and culture systems directly deliver Cas9/sgRNA ribonucleoproteins, providing a fast turnaround for gene editing while minimizing off-target effects compared to vector-based approaches.

    Furthermore, our complete systems circumvent a major bottleneck in the hiPSC editing process: survival and expansion of edited single clones. Traditionally, the establishment of a clonal population from hiPS cells grown as colonies results in cell death or premature differentiation. However, Cellartis’ kits contain a defined culture system that promotes monolayer growth, overcoming the challenges of colony-based culture by allowing efficient single-cell passaging and promoting expansion of highly pluripotent, edited hiPSCs.

    • Efficient gene editing in hiPSCs—use electroporation or gesicles to deliver Cas9 protein and sgRNA with no genomic integration and reduced off-target effects
    • Maintenance of pluripotency and stable karyotype from start to finish—hiPSCs maintain high levels (>90%) of pluripotency markers and a normal, stable karyotype throughout editing, single-cell cloning, and expansion
    • Flexibility to perform gene editing experiments your way—choose from two different all-in-one editing and single-cell-cloning solutions, or use the culture media kit to perform efficient single-cell cloning following your own editing experiments

    A new standard for stranded total RNA-seq from picogram inputs

    We are pleased to announce the release of the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Pico v2), an updated version of our industry-leading solution for analysis of challenging samples. The Pico v2 kit is designed to generate Illumina-ready sequencing libraries from 250 pg–10 ng of high-quality, partially degraded, or low-quality mammalian total RNA, including RNA obtained from FFPE samples. Like its predecessor, the Pico v2 kit retains strand-of-origin information and employs a proprietary technology for the removal of ribosomal cDNA following reverse transcription and PCR amplification. Updates included in the Pico v2 kit provide improved sequencing performance, resulting in higher percentages of clusters passing filter (%PF) without the addition of PhiX, thereby reducing sequencing costs. The Pico v2 kit also includes a new buffer formulation that improves sample handling during library purification.

    • Generates high-quality Illumina-ready sequencing libraries from 250 pg–10 ng mammalian total RNA inputs of any quality, including RNA obtained from FFPE or LCM samples
    • Provides improved performance and sequencing metrics on all Illumina platforms, including higher %PF, more transcripts identified, and lower duplicate rates
    • User-friendly workflow can be completed in ~6 hours
    • Available in 12, 48, 96, and 192-reaction sizes

    High-quality human embryonic stem cell kits for expansion and differentiation into all three germ layers

    Cellartis human embryonic stem (ES) cell line kits contain human ES cells that have been ethically derived under feeder-free conditions, then banked and characterized according to the highest industry standards. The human ES cells are adapted to the Cellartis DEF-CS culture system, an easy-to-use, complete culture system for pluripotent cell expansion in a defined, feeder-free environment that maintains high levels of pluripotency. The reproducible nature of the DEF-CS system coupled with the stable high growth rate of these stem cell lines is ideal for small- or large-scale expansion, as well as any major downstream applications.

    • Cells are extensively analyzed for purity, recovery after thawing, absence of mycoplasma and bacteria, expression of stemness markers, absence of differentiation markers, and expected karyotype
    • Cellartis hES cell lines are available from four donors and are supplied from fully characterized cell banks
    • The derivation process follows all applicable laws in Sweden and the EU and is approved by a regional ethical authority

    Trypsin mini spin columns for rapid, complete protein digestion

    Capturem Trypsin digests proteins completely in less than one minute at room temperature, allowing for an uninterrupted proteomic analysis workflow. It consists of membrane-immobilized trypsin in a mini spin column format which is based on our novel Capturem technology. Capturem Trypsin offers the following advantages over in-solution trypsin digestion:

    • Digestion of protein samples in less than one minute instead of overnight
    • More efficient and complete protein digestion
    • Fewer autolyzed trypsin fragments and protein modifications
    • More effective peptide and protein identification in MS analysis

    Move beyond traditional immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) workflows

    Although IPs and Co-IPs are still frequently used to study protein interactions, the traditional purification methods used for isolation of the antigen-protein complexes can be time-consuming. The Capturem IP & Co-IP Kit combines high-capacity, membrane-based Capturem Protein A columns with optimized lysis, wash, and elution buffers for a complete, easy-to-use solution.

    • Isolates antibody-bound protein complexes in just 15 minutes—from incubation of antibody with sample through elution
    • Reduces the chances of aggregation, loss of activity, and complex disassociation
    • Includes Capturem Protein A columns and optimized IP buffers

    CRISPR/Cas9 editing made faster by protein delivery

    Guide-it Recombinant Cas9 (Electroporation-Ready) is the newest addition to our line of products for CRISPR/Cas9 gene editing experiments. Delivery of Cas9-sgRNA ribonucleoprotein complexes provides a fast turnaround for gene-editing experiments while minimizing the likelihood of off-target effects compared to vector-based approaches.

    • Works well in hard-to-transfect cell lines
    • Minimizes off-target effects—better than plasmid-based delivery systems 
    • Enables highly efficient editing when combined with our Guide-it Complete sgRNA Screening System
    • Provides a faster turnaround for gene editing experiments

    New Xeno-Free and GMP-Grade Stem Cell Media Products to Accelerate Discovery

    Moving human pluripotent cell-based therapies from the lab to the clinic involves jumping over key hurdles in the process, and our Cellartis brand xeno-free media products are designed with these challenges in mind. Stemming from more than 15 years of expertise in pluripotent cell generation and differentiation, our new additions to the xeno-free media product family aim to maximize the potential of your cell therapy and ultimately influence human health.

    • Our original xeno-free medium promotes robust 2D monolayer expansion and is suitable for preclinical and research applications.
    • Our NEW xeno-free 3D spheroid culture medium is optimized for scale-up of iPSCs as 3D spheroids for downstream directed differentiation—5 x 109 cells in a 1 liter bioreactor within 3–4 passages.
    • Our NEW xeno-free GMP-grade basal medium is manufactured with clinical-quality materials and traceable production processes, and is ideal for small- or large-scale production of pluripotent cells for downstream use in clinical research settings.

    Efficient T-Cell Culture and Expansion Medium

    Promoting robust T-cell expansion is an important prerequisite for basic and preclinical studies, including adoptive cell therapy studies. GT-T551 Culture medium, 1L Bottle is ideally suited for expansion of T cells, and is recommended in our protocol for anti-CD3/IL-2 T-cell activation in conjunction with RetroNectin. This protocol results in an expanded cell population that shows a higher proportion of naïve T cells than anti-CD3 antibody stimulation alone. This T cell medium is:

    • Supplemented with human serum albumin, human insulin, L-glutamine, and streptomycin
    • Endotoxin tested (endotoxin <0.3 EU/ml)
    • Compatible with CultiLife Culture Bags for large-scale expansion of T cells
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