Chromatin immunoprecipitation (ChIP) experiments often yield very small amounts of DNA. Unfortunately, most next-generation sequencing (NGS) library preparation methods use ligation to add sequencing adapters and require a larger amount of DNA. The DNA SMART ChIP-Seq Kit eliminates this problem; instead of using ligation, Illumina® sequencing adapters are added directly to low-input ChIP DNA samples via a novel, SMART-based template switching technology. Eliminating ligation from library preparation means only a single, combined size selection and clean-up step is needed, leading to higher yield and a simpler workflow.
Flexible DNA inputs: 100 pg–10 ng of either ssDNA or dsDNA, or total DNA from 10,000–1,000,000 cell ChIP experiments
Illumina-ready sequencing libraries in around 4 hours: Illumina indexes and adaptors incorporated into the workflow
Excellent sequencing metrics: High non-redundant rate, high reproducibility between different sample inputs, and high overlap with data generated by the ENCODE project
The Library Quantification Kit provides a highly-sensitive, qPCR-based quantification method for measuring DNA libraries for Illumina-based NGS. The kit contains all of the reagents necessary for absolute quantification of sequencing libraries containing Illumina adapters. Use this kit prior to sequencing to ensure optimal loading concentration, thereby reducing cluster density variability and improving sequencing results.
PCR, clone, PCR, sequence―with the Guide-it Indel Identification Kit, characterizing mutations introduced by genome editing is as simple as that. The kit includes all of the components needed to amplify, clone, and prepare modified target sites for DNA sequence analysis. Plus, with the ultra-streamlined protocol that includes time-saving Terra PCR Direct Polymerase and the innovative In-Fusion cloning system, reliable sequencing results can be obtained in a matter of days.