In-Fusion HD Plus: The trustworthy solution for all your cloning projects

The In-Fusion HD Cloning Plus kits include all the reagents you need for highly efficient and reliable cloning—In-Fusion HD cloning premix, a high-fidelity PCR enzyme, and competent cells with a transformation efficiency of >5 X 10⁸ cfu/µg.

• Clone directly into any vector
• Guaranteed >95% cloning efficiency
• Seamless construction
• Get everything you need to clone, in a one-box value pack

Magnetic beads for DYKDDDDK-tagged protein purification

The Magnetic DYKDDDDK Immunoprecipitation Kit offers a simple, rapid method for purifying or immunoprecipitating FLAG-tagged fusion proteins from cell lysates using magnetic beads crosslinked to monoclonal anti-DYKDDDDK antibody. The kit also includes the DYKDDDDK Immunoprecipitation Buffer Set and the ProteoGuard EDTA-Free Protease Inhibitor Cocktail.

• Quick, easy magnetic separation
• Yields highly pure denatured proteins
• Perform high-throughput protein-protein interaction studies

Find insertion sites or upstream promoter sequences using long distance PCR

The Universal GenomeWalker 2.0 kit contains all the reagents needed for PCR-based “walking” in the genomic DNA of any species. Universal GenomeWalker 2.0 makes it possible to:

• Obtain promoters or regulatory sequences
• Map intron/exon junctions
• Determine integration sites for transposons and viruses
• Confirm genome modifications performed using zinc finger nucleases, TAL effector nucleases, or other methods

Magnetic beads for microscale his-tagged protein purification

His60 Ni Magnetic Beads offer a rapid and simple method for purifying small amounts of his-tagged protein.The modified Ni-IDA chemistry of His60 Ni makes this resin one of the best on the market in terms of capacity. The beads are designed for the microscale purification of high and low molecular weight recombinant his-tagged proteins under native or denaturing conditions, when placed on a magnetic separator.

Antibodies for iDimerize systems

iDimerize inducible protein expression systems allow you to control protein-protein interactions by using a small molecule ”dimerizer” ligand to induce dimerization of protein(s) of interest fused to Dmr tags. Dmr antibodies let you monitor the expression levels of your Dmr fusion proteins and are available for all three types of Dmr tags. The DmrB Monoclonal Antibody is the companion tool for iDimerize Inducible Homodimer Systems. The DmrA Monoclonal Antibody and DmrC Polyclonal Antibody are designed for use with any iDimerize Inducible Heterodimer Expression System and the iDimerize Regulated Transcription System.

Sensitive detection of Metridia luciferase

The Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase. It is ideal for use with the Ready-To-Glow Secreted Luciferase System, a live-cell assay which is based on the secreted Metridia luciferase reporter.

• Detects as little as 20 ng of Metridia luciferase using Western blot analysis
• Recognizes Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase

Precise, rapid control of protein-protein interactions

Protein-protein interactions trigger signal transduction cascades, induce protein accumulation at specific cellular locations, and control a host of other biological processes. One challenge in artificial control of protein-protein interactions is that proteins can dimerize simply because they are in close physical proximity—particularly if they are membrane proteins.

Now you can eliminate these unwanted events: we’ve combined iDimerize and Tet-On 3G technologies in the iDimerize Inducible Homodimer and Heterodimer Systems (with Tet-On 3G) to give you two-tiered control of protein behaviors:

1. First, use doxycycline to optimize the proteins’ expression to physiologically-relevant levels
2. Then induce dimerization by adding the dimerizer ligand to your culture medium

High-quality Tet System Approved Fetal Bovine Serum

Only Clontech’s functionally tested Tet System Approved Fetal Bovine Serum has been certified to ensure optimum induction with all tetracycline-inducible gene expression systems—unlike other serum products, which may contain tetracycline contaminants that can dramatically affect the inducible regulation of Tet Systems.

We offer four different versions of Tet System Approved FBS, which are now available in larger sizes:

• US-Sourced FBS—premium serum collected in the United States
• Australia-Sourced FBS—premium serum collected in Australia
• ES Cell Qualified FBS—premium serum collected in the United States and qualified for use in embryonic stem cells
• Tet System Approved FBS—economical alternative from non-US, USDA-approved sources

Rapid dissociation of protein dimers with the B/B Washout Ligand

Looking for the next step in your iDimerize protein interaction studies? B/B Washout Ligand dissociates iDimerize protein interactions rapidly and completely. Simply replacing medium containing B/B Homodimerizer with medium without B/B Homodimerizer has only a minor dissociative effect.