Successful recombinant his-tagged protein purification is dependent on an effective expression and purification protocol. At Clontech, we have developed reagents and kits that are designed to address all recombinant his-tagged protein purification needs. Whether you are performing small- or large-scale purification, or are interested in cell lysis/extraction and detection of your recombinant his-tagged protein, you can choose from our top performing products.
There are specific times when it’s best to use cobalt-based resins, and other times when a nickel-based resin will do the best job. It all depends whether you require the highest purity (choose TALON cobalt resin) or the highest yield (choose His60 Nickel resin).