Sign up to watch our In-Fusion cloning series. These videos are now available:

• Simpify Cloning with the In-Fusion Cloning System
• Get Easy Deletions with the Mutagenesis Cloning Kit

• Express proteins at high levels: 1 to 500 mg of protein per liter of culture
• Obtain structure, activity, and immunological reactivity similar to the naturally occurring protein
• >90% of the viruses produced by the transfected cells carry the target protein

Guest lecture by Yi Gong, Ph. D., Laboratory of Cell Biology and Genetics, Rockefeller University.

• The Matchmaker Gold System minimizes background and false positives, and provides more yeast two-hybrid (Y2H) interactions
• Normalized libraries are the simplest, most straightforward method of screening a Y2H library
• DIY libraries create library aliquots for hundreds of yeast two-hybrid screens in less than a week
• See a typical Y2H experimental workflow

• SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that enables generation of high-quality cDNA from as little as 1–2 ng total RNA starting material.
• SMART maintains the complexity of the original RNA—obtain a true representation of your sample.

Analyzing protein function is a key focus in cell biology research. The ProteoTuner systems make it possible to investigate the function of a specific protein of interest directly—by rapidly changing the abundance of the protein itself. Applications highlighted in this webinar include:

• Observing actin polymerization
• Controlling ion channel function
• Regulating zinc finger nuclease toxicity

• What are the different types of promoter reporters?
• How to choose the reporter that will maximize your data
• How can you benefit from using two reporters?
• Your promoter reporter choices

• Why should I purify recombinant proteins? Why are his-tags a good choice?
• What protein expression system should I use?
• When are cobalt resins (like TALON) good to use? What about nickel resins like His60 Ni?