Learn about In-Fusion PCR Cloning products and discover how you can use the system to efficiently construct any clone you desire, without subcloning.

This webinar features Dr. Shujun Luo, Senior Staff Scientist at Illumina, who provides a detailed overview of the SMARTer Ultra Low RNA Kit for Illumina Sequencing. This newly developed system enables researchers to analyze previously inaccessible samples.

• Express proteins at high levels: 1 to 500 mg of protein per liter of culture
• Obtain structure, activity, and immunological reactivity similar to the naturally occurring protein
• >90% of the viruses produced by the transfected cells carry the target protein

Guest lecture by Yi Gong, Ph. D., Laboratory of Cell Biology and Genetics, Rockefeller University.

• The Matchmaker Gold System minimizes background and false positives, and provides more yeast two-hybrid (Y2H) interactions
• Normalized libraries are the simplest, most straightforward method of screening a Y2H library
• DIY libraries create library aliquots for hundreds of yeast two-hybrid screens in less than a week
• See a typical Y2H experimental workflow

Clontech’s Lenti-X high-titer lentiviral packaging systems generate lentiviral titers that are vastly superior to most other commercially available lentiviral packaging systems. At titers of 10⁷–10⁸ infectious units (IFU) per ml, you can often transduce an entire dish of target cells using only 10 μl of unconcentrated lentiviral packaging cell supernatant.

• SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that enables generation of high-quality cDNA from as little as 1–2 ng total RNA starting material.
• SMART maintains the complexity of the original RNA—obtain a true representation of your sample.

Analyzing protein function is a key focus in cell biology research. The ProteoTuner systems make it possible to investigate the function of a specific protein of interest directly—by rapidly changing the abundance of the protein itself. Applications highlighted in this webinar include:

• Observing actin polymerization
• Controlling ion channel function
• Regulating zinc finger nuclease toxicity

• What are the different types of promoter reporters?
• How to choose the reporter that will maximize your data
• How can you benefit from using two reporters?
• Your promoter reporter choices

• Why should I purify recombinant proteins? Why are his-tags a good choice?
• What protein expression system should I use?
• When are cobalt resins (like TALON) good to use? What about nickel resins like His60 Ni?