For Overview of Technology Licensing Program
Clontech Laboratories has a broad, robust technology portfolio. Our innovations have been licensed for use by entities of all sizes, types, and stages. Fluorescent Protein tools comprise a key technology area with powerful applications. We also offer several other versatile technologies in the areas of molecular biology and protein purification. Partner with us and put our innovation to work for you.
Featured Technology Available for Licensing:
Fluorescent proteins are powerful tools for investigating protein expression and cellular events noninvasively, in real time, and without need for cofactors or substrates. A single fluorescent protein is useful for tagging and visualizing one protein, as a transfection control, or as a marker for cell sorting and clone isolation. Two or more Fluorescent Proteins used simultaneously allow multiplexing experiments, co-localization studies, and sorting of complex cell populations. Under the Clontech Out-Licensing program, fluorescent protein license agreements are available for a range of applications.
Our Living Colors Fluorescent Proteins are derived from a wide array of sea organisms and cover a broad spectrum of excitation and emission wavelengths, giving rise to 6 distinct colors: far red, red, orange, yellow, green, and cyan. As a long-standing leader in the fluorescent protein field, Clontech Laboratories Inc. has commercialized Living Colors fluorescent proteins for use in many applications in basic research and drug development. Living Colors Fluorescent Proteins have been integrated into a variety of assays and products.
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- Research Use: Non-exclusive research use licenses are available for uses including basic research, production of transgenic organisms, and HTS or HCS screen development.
- Commercial Use: Depending on the specific field of interest, both exclusive and non-exclusive commercial licenses are available for uses including third-party contract services, QC and QA, manufacturing, and transgenics.
- If you are a for-profit entity, please contact a Clontech Laboratories representative (using the inquiry form below) regarding licensing terms for research or commercial use.If you are a not-for-profit entity wishing to obtain a research-only Fluorescent Protein license agreement, please click here for the applicable terms. If you are a not-for-profit entity but wish to receive a commercial license, please contact a Clontech representative.
In addition to Fluorescent Protein technology, Clontech out-licensing agreements are available for several other technologies such as our MSCV Retroviral Vectors, Suppression PCR, TALON Resin, and histidine-containing tags for purification of polyhistidine-tagged proteins. To learn more about available licenses, please contact the Clontech Business Development group at 650.919.7320 or submit your request using the form at the right-hand side of this page.
MSCV Retroviral Vectors
Murine Stem Cell Virus (MSCV) vectors were derived from the Murine Embryonic Stem Cell Virus (MESV) and the LN retroviral vectors. Upon transfection into a packaging cell line pMSCVhyg transiently expresses (or integrates and stably expresses) a transcript containing the extended viral packaging signal psi+, the hygromycin resistance gene, and a gene of interest. The vectors achieve stable, high-level gene expression in hematopoietic and embryonic stem cells through a specifically designed 5' long terminal repeat (LTR). This LTR is from the murine stem cell PCMV virus and differs from the MoMuLV LTR used in other retroviral vectors by several point mutations and a deletion. These changes enhance transcriptional activation and prevent transcriptional suppression in embryonic stem and embryonal carcinoma cells. Thus, the LTR drives high-level constitutive expression of a target gene in stem cells or other mammalian cell lines when cloned into the multiple cloning site downstream of the 5' LTR. The murine phosphoglycerate kinase (PKG) promoter (PPKG) controls expression of the hygromycin resistance gene (Hygr) for antibiotic selection in eukaryotic cells. PMSCVhyg also contains the pUC origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.
TALON Metal Affinity Resin is a durable immobilized metal affinity chromatography (IMAC) resin consisting of a tetradentate chelator charged with cobalt. This resin has a remarkable affinity and specificity for His-tagged proteins. The stable chelation of the Co2+ ion combined with the specificity of the TALON reactive core deliver unmatched purity. TALON Metal Affinity Resin is compatible with all commonly used IMAC reagents and allows protein purification under native or denaturing conditions, and in the presence of beta-mercaptoethanol.