Looking for EGFP Vectors? Upgrade to AcGFP1 Vectors

AcGFP1 has 94% homology to EGFP at the amino acid level.
AcGFP1 and EGFP have similar excitation and emission maxima.
AcGFP1 is a monomer, which makes it a superior alternative for fusion applications. EGFP has been reported to form dimers (1, 2).

Another alternative to EGFP is ZsGreen1, a very bright, tetrameric green fluorescent protein which is an ideal reporter. ZsGreen1 has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells (3).

Old EGFP Vector	Suggested Replacement	Application
EGFP Vector Conversion
pBI-EGFP Tet Vector	Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1)	Simultaneous, equivalent Tet-inducible expression of ZsGreen1 and your protein of interest from the same bidirectional promoter.
pCMS-EGFP Vector	pIRES2-AcGFP1 Vector	Express your protein of interest and AcGFP1 from the same transcript.
pCRE-D2EGFP Vector	CRE DD Green Reporter System	Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.
pD1EGFP-N1 Vector	pZsGreen1-DR Vector	Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.
pD2EGFP Vector	pZsGreen1-DR Vector	Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.
pD2EGFP-1 Vector	pZsGreen1-DR Vector	Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.
pD2EGFP-N1 Vector	pZsGreen1-DR Vector	Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.
pD4EGFP-N1 Vector	pZsGreen1-DR Vector	Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.
pD4EGFP-Bid Vector	pDsRed2-Bid Vector	Monitor Bid translocation in living cells with DsRed2.
pDNR-EGFP Donor Reporter Vector	pAcGFP1-C In-Fusion Ready Vector pAcGFP1-N In-Fusion Ready Vector	Prelinearized vectors for simple, one-step In-Fusion cloning of your gene of interest fused to AcGFP1.
pEGFP Vector	pAcGFP1 Vector AcGFP1 Vector Set	Express AcGFP1 in bacterial systems.
pEGFP-1 Vector	pAcGFP1-1 Vector	Monitor the activity of promoters and promoter/enhancer combinations with an AcGFP1 reporter.
pEGFP-Actin Vector	pAcGFP1-Actin Vector	Visualize the actin cytoskeleton with AcGFP1.
pEGFP-C Vector Set	pAcGFP1-C Vector Set	Express and visualize a protein of interest fused to AcGFP1.
pEGFP-C1 Vector	pAcGFP1-C1 Vector AcGFP1 Vector Set pAcGFP1-C In-Fusion Ready Vector	Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning.
pEGFP-C2 Vector	pAcGFP1-C2 Vector	Express and visualize a protein of interest fused to AcGFP1.
pEGFP-C3 Vector	pAcGFP1-C3 Vector	Express and visualize a protein of interest fused to AcGFP1.
pEGFP-Endo Vector	pAcGFP1-Endo Vector	Monitor intracellular membrane traffic with AcGFP1.
pEGFP-F Vector	pAcGFP1-F Vector pAcGFP1-F Hyg Vector	Label the inner leaflet of the plasma membrane with AcGFP1 and select with either G418 or hygromycin.
pEGFP-N Vector Set	pAcGFP1-N Vector Set	Express and visualize a protein of interest fused to AcGFP1.
pEGFP-N1 Vector	pAcGFP1-N1 Vector AcGFP1 Vector Set pAcGFP1-N In-Fusion Ready Vector	Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning.
pEGFP-N2 Vector	pAcGFP1-N2 Vector	Express and visualize a protein of interest fused to AcGFP1.
pEGFP-N3 Vector	pAcGFP1-N3 Vector	Express and visualize a protein of interest fused to AcGFP1.
pEGFP-Peroxi Vector	pDsRed2-Peroxi Vector	Label peroxisomes with DsRed2.
pEGFP-Tub Vector	pAcGFP1-Tubulin Vector	Visualize the microtubule network with AcGFP1.
pHygEGFP Vector	pAcGFP1-Hyg-N1 Vector pAcGFP1-Hyg-C1 Vector pAcGFP1-Mem Hyg Vector pAcGFP1-F Hyg Vector	Establish stable cell lines coexpressing AcGFP1 by selecting with hygromycin. Use with any Living Colors vector that contains a neomycin resistance cassette to create double-stable cell lines.
pIRES2-EGFP Vector	pIRES2-AcGFP1 Vector	Express your protein of interest and AcGFP1 from the same transcript.
pIRES-EGFP Vector	pIRES2-AcGFP1 Vector	Express your protein of interest and AcGFP1 from the same transcript.
pLEGFP-C1 Retroviral Vector	pRetroQ-AcGFP1-C1 Vector	Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells.
pLEGFP-N1 Retroviral Vector	pRetroQ-AcGFP1-N1 Vector	Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells.
pLP-EGFP-C1 Acceptor Vector	pAcGFP1-C In-Fusion Ready Vector	Prelinearized vector for simple, one-step In-Fusion cloning of your gene of interest fused to AcGFP1.
pLPS-3'EGFP Acceptor Vector	pAcGFP1-C In-Fusion Ready Vector pACGFP1-N In-Fusion Ready Vector	Prelinearized vectors for simple, one-step In-Fusion cloning of your gene of interest fused to AcGFP1.
pNeoEGFP Vector	pAcGFP1-N1 Vector pAcGFP1-N2 Vector pAcGFP1-N3 Vector pAcGFP1-N Vector Set pAcGFP1-C1 Vector pAcGFP1-C2 Vector pAcGFP1-C3 Vector pAcGFP1-C Vector Set AcGFP1 Vector Set	Establish stable cell lines expressing AcGFP1 or AcGFP1 fusion proteins by selecting with G418.
pNF-KB-D2EGFP Vector	NFkappaB DD Green Reporter System	Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.
pREVTRE2-d1EGFP Vector	Tet-On 3G Inducible Expression System (with ZsGreen1)	Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1.
pTRE-D2EGFP Vector	Tet-On 3G Inducible Expression System (with ZsGreen1)	Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1.
pTRE-Tight-EGFP Vector	Tet-On 3G Inducible Expression System (with ZsGreen1)	Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1.
Signaling Probe CREB-EGFP	CRE DD Green Reporter System	Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.

References

Jain, R. K. et al. (2001) Biochem. J. 360(Pt. 3):645–649.
Chen, Y. et al. (2002) Biophys. J. 82(1):133–144.
Bell, P. et al. (2007) J. Histochem. Cytochem. 55(9):931–939.


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