Enrich for Cells of Interest with CherryPicker Systems
CherryPicker cell capture was used to enrich for MEK-expressing cells, as indicated by ERK1 levels (compare the “Before capture” and “After capture” western blot data).
HEK-293 cells were transiently transfected with the CherryPicker-IRES expression vector encoding a constitutively active mutant of MEK (MEK-CA). Transfected cells were mixed 1:10 with untransfected cells to reduce the number of MEK-expressing cells in the population. This mixed cell population was either:
- Immediately subjected to western blot analysis (“Before capture”; Lanes 2–4), or
- Enriched for MEK-expressing cells by performing CherryPicker capture and enrichment before western blotting (“After capture”; Lane 5)
Western blot analysis used a phosphospecific antibody against phosphorylated ERK1. To allow for normalization, the western blot was also tested using an antibody against the ubiquitously expressed GAPDH.
Visit the Documents tab of the CherryPicker product page to download the User Manual and other documentation.
