Effective Gene Silencing
Many siRNA transfection reagents were designed for plasmid delivery and then sold for siRNA transfection, so they aren’t really optimized for siRNA transfection. The Xfect siRNA Transfection Reagent was specifically created for siRNA delivery—so it provides better results.
High Viability
The Xfect siRNA Transfection Reagent was developed to maintain a very low cytotoxicity profile while delivering high rates of gene silencing. Don’t hesitate to transfect—your cells will survive it! (We routinely measure 100% viability for HeLa cells.)
Simple Protocol
Many siRNA transfection reagents require you to pre-test different ratios of siRNA oligo to reagent. But not the Xfect siRNA Transfection Reagent—we’ve pre-determined the best ratio for any size of plate or dish, so you can skip the preliminaries and get started with your experiment!
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Take the guesswork out of siRNA transfection with the Xfect reagent
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| Culture vessel |
Surface area/well |
Growth medium |
Xfect reaction buffer volume |
siRNA |
Xfect siRNA transfection polymer volume |
| 96-well plate |
0.3 cm2 |
100 µl |
2 x 20 µl |
4.5 pmol |
0.6 µl |
| 24-well plate |
2 cm2 |
500 µl |
2 x 50 µl |
30 pmol |
4 µl |
| 12-well plate |
4 cm2 |
1 ml |
2 x 80 µl |
60 pmol |
8 µl |
| 6-well plate |
10 cm2 |
2.5 ml |
2 x 200 µl |
150 pmol |
20 µl |
| 60-mm dish |
20 cm2 |
5 ml |
2 x 400 µl |
300 pmol |
40 µl |
| 10-cm dish |
60 cm2 |
15 ml |
2 x 1.2 ml |
900 pmol |
120 µl |
Visit the Documents tab of the Xfect siRNA Transfection Reagent product page to download the User Manual and other documentation.
