His60 Ni Superflow Resin has better purity among nickel resins (outperforming Ni-NTA resins), as well as low metal ion leakage. Its modified Ni-IDA chemistry makes His60 nickel resin one of the best on the market when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins:
High capacity (60 mg/ml)
Low metal ion leakage
His60 nickel resin allows one-step purification of any recombinant his-tagged protein from any expression system, under native or denaturing conditions (compatible with 6xHis-tags and HN-tags). His60 Ni Superflow resin can be used to purify recombinant his-tagged proteins from any expression system, including baculovirus, mammalian cells, yeast, and bacteria.
His60 Nickel Resin Provides Higher Purity of His-Tagged Proteins than Ni-NTA Resin
Better purity was obtained when using His60 Ni Superflow resin to purify recombinant his-tagged protein. Ni-NTA Superflow resin provided lower purity and yield. 6xHN-AcGFP1 was expressed in E. coli. The protein was purified from equivalent amounts of the same sample, using His60 Ni Superflow Resin purification (left) and Competitor Q’s Ni-NTA Resin purification (right) according to each manufacturer’s protocol. Lane M: Molecular weight marker. Lane OS: Original sample. Lane FT: Flowthrough. Lanes W1 and W2: Wash. Lane E1–E4: Eluted 6xHN-AcGFP1 fractions.
His60 Nickel Superflow Resin is a high-capacity Ni-IDA resin for the efficient purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. His60 Ni Resin is compatible with batch/gravity flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing, and it outperforms Ni-NTA resins (in capacity and purity). The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses. His60 Ni Superflow resin is available in a variety of sizes and as prepacked His60 Ni Gravity Columns. Using the same resin for small-scale and large-scale purifications means that you do not need to re-optimize conditions when you move to large-scale purifications. The purified recombinant his-tagged proteins can be used for protein labeling, immunization to produce antibodies, or in animal studies.
The purification of recombinant his-tagged proteins consists of cell lysis/extraction, binding, washing, and elution. His60 Nickel resin allows one-step protein purification under either native or denaturing conditions. Purified recombinant his-tagged proteins can be eluted with imidazole, or by a reduction in pH.
Purify his-tagged proteins under native or denaturing conditions with His60 Ni Resin. His60 Ni resin is compatible with batch/gravity-flow applications, as well as with the major automated liquid chromatography systems or manual syringe processing.