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Support >  Applications >  Tagged_Protein_Purification >  Ni-NTA_Resin_vs_His60

His60 Nickel Resin Beats Ni-NTA Resin for His-Tag Purification

His60 Nickel Resin Beats Ni-NTA Resin for His-Tag Purification

What is His60 resin? | How His60 resin works | Applications | Related products

His60 Ni Superflow Resin has better purity among nickel resins (outperforming Ni-NTA resins), as well as low metal ion leakage. Its modified Ni-IDA chemistry makes His60 nickel resin one of the best on the market when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins:

  • High capacity (60 mg/ml)
  • Low metal ion leakage
  • High purity

His60 nickel resin allows one-step purification of any recombinant his-tagged protein from any expression system, under native or denaturing conditions (compatible with 6xHis-tags and HN-tags). His60 Ni Superflow resin can be used to purify recombinant his-tagged proteins from any expression system, including baculovirus, mammalian cells, yeast, and bacteria.

His60 Nickel Resin Provides Higher Purity of His-Tagged Proteins than Ni-NTA Resin

Better his tag protein purity and yield with His60 Nickel Resin than Ni-NTA Resin.

Better purity was obtained when using His60 Ni Superflow resin to purify recombinant his-tagged protein. Ni-NTA Superflow resin provided lower purity and yield. 6xHN-AcGFP1 was expressed in E. coli. The protein was purified from equivalent amounts of the same sample, using His60 Ni Superflow Resin purification (left) and Competitor Q’s Ni-NTA Resin purification (right) according to each manufacturer’s protocol. Lane M: Molecular weight marker. Lane OS: Original sample. Lane FT: Flowthrough. Lanes W1 and W2: Wash. Lane E1–E4: Eluted 6xHN-AcGFP1 fractions.

What is His60 Resin?

His60 Nickel Superflow Resin is a high-capacity Ni-IDA resin for the efficient purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. His60 Ni Resin is compatible with batch/gravity flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing, and it outperforms Ni-NTA resins (in capacity and purity). The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses. His60 Ni Superflow resin is available in a variety of sizes and as prepacked His60 Ni Gravity Columns. Using the same resin for small-scale and large-scale purifications means that you do not need to re-optimize conditions when you move to large-scale purifications. The purified recombinant his-tagged proteins can be used for protein labeling, immunization to produce antibodies, or in animal studies.

How Does His60 Resin Work?

The purification of recombinant his-tagged proteins consists of cell lysis/extraction, binding, washing, and elution. His60 Nickel resin allows one-step protein purification under either native or denaturing conditions. Purified recombinant his-tagged proteins can be eluted with imidazole, or by a reduction in pH.

Workflow for purifying his-tagged proteins with His60 Ni resin (60 mg protein/ml resin).

Purify his-tagged proteins under native or denaturing conditions with His60 Ni Resin. His60 Ni resin is compatible with batch/gravity-flow applications, as well as with the major automated liquid chromatography systems or manual syringe processing.

Applications

  • Use His60 Ni Resin to obtain better purification of His-tagged proteins than Ni-NTA resin can provide.
  • Purify recombinant his-tagged proteins from any expression system, including baculovirus, mammalian cells, yeast, and bacteria.
  • Use purified proteins for any of the following applications:
    • Protein labeling
    • Immunization to produce antibodies
    • Animal studies

We Also Recommend:

  • His60 Ni FPLC Cartridges
    • Prefilled 1 ml and 5 ml cartridges for his-tagged protein purification on any LC system, such as ÄKTA or FPLC, or for manual purification using a syringe.
  • TALON Cobalt Resin
    • TALON provides the very highest purity for his-tagged proteins, when compared to any nickel resin.
  • His-Tag Detection Antibodies
    • Use highly sensitive antibodies to detect his-tagged recombinant proteins in Western blot, ELISA, and immunocytohistochemical assays.
  • ProteoGuard Protease Inhibitor Cocktail
    • Use this protease inhibitor cocktail to suppress proteolysis in all your cell lysates. We recommend using ProteoGuard with all our protein purification products.

 
 
Products
Cat. # Product Package Size Price # of Units Select
635659 His60 Ni Superflow Resin 10 mL $93.00
635660 His60 Ni Superflow Resin 25 mL $185.00
635661 His60 Ni Superflow Resin 4 x 25 mL $711.00
635662 His60 Ni Superflow Resin 250 mL $1,700.00
635663 His60 Ni Superflow Resin 2 x 250 mL $2,936.00
635664 His60 Ni Superflow Resin 4 x 250 mL $5,562.00
635665 His60 Ni Buffer Set 20 Purifications $216.00
635676 His60 Ni Superflow™ Resin & xTractor Buffer Bundle 20 Purifications $202.00
635677 His60 Ni Superflow™ Resin & Buffer Set Bundle 20 Purifications $299.00
 

 

 

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