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Support >  Applications >  PCR >  Long_PCR

High Fidelity PCR

PCR Applications

Long PCR

Go the Distance

When do you need it?

Long PCR refers to instances where DNA templates over 8.0 kb in length (i.e. genomic DNA) are amplified.

How is it achieved?

The two factors critical to long PCR are enzyme fidelity and processivity. Thermostable Taq polymerase, for example, is highly processive but lacks 3'-5' exonuclease (proofreading) activity. Enzyme blends of Taq with high fidelity polymerases (i.e. pfu) can achieve the level of performance required for amplifying long templates. External factors like template sequence, buffer composition, and reaction conditions are also important for long PCR.

What makes our PCR polymerases the best?

We offer high performance enzyme mixes for both non-complex and GC-rich long PCR applications.

  • Advantage Genomic LA Mix is an enzyme blend of full-length, thermostable Taq DNA Polymerase, a small amount of proofreading enzyme, and optimized buffer components for the perfect balance of fidelity, length, specificity, and yield. It provides 6.5-fold higher fidelity than wild-type Taq and amplifies non-complex targets up to 43 kb.
  • Advantage GC Genomic LA Mix includes an additional GC-Melt buffer system, designed specifically for efficient amplification of virtually all GC-rich sequences, up to 90% GC content, while maintaining the ability to amplify long template sequences with fidelity.

How to pick the right enzyme?

Polymerase Application Fidelity* Amplicon size (kb) Human/plasmid/cDNA Max GC content Hot Start Cloning compatibility  
Advantage Genomic LA Long PCR 6.5X <8.030.0/<43.0/<10.0 60% Yes TA Buy now
Advantage GC Genomic LA Long PCR, GC-rich PCR 6.5X <8.030.0/<20.0/<10.0 90% Yes TA Buy now
* Relative fidelities are represented as fold improvements over Titanium Taq DNA Polymerase, which has an error rate of 1 mismatch per kb of synthesized DNA.
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