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Direct PCR

PCR Applications

Direct PCR

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When do you need it?

Direct PCR allows for amplification directly from the tissue source or crude extracts, without the need for DNA purification. Omitting the multi-step process for preparing genomic DNA helps prevent sample loss, saves time, and reduces cost. Direct PCR vastly simplifies genotyping experiments, especially when the source material is limited.

How is it achieved?

Direct PCR relies on modified polymerases and buffer systems that enable DNA amplification in the presence of PCR inhibitors, growth serum, and other source material components.

Terra PCR Direct Solution Polymerase Mix Red Dye Premix Genotyping Kit FFPE Kit FTA Card Kit
Features: General purpose; suitable for most applications Premix with convenient loading All-in-one kit; most robust performance Application-specific kit Application-specific kit
Components: Buffer
[Mg2+, dNTPs]
Premix
[Mg2+, dNTPs,
 
Polymerase Mix,
 
Loading Dye]
 
Buffer
[Mg2+, dNTPs]
Buffer
[Mg2+, dNTPs]
Buffer
[Mg2+, dNTPs]
Polymerase Mix Polymerase Mix Polymerase Mix Polymerase Mix
  Loading Dye    
    Extraction Buffer* Extraction Buffer  
Proteinase K Proteinase K Proteinase K Proteinase K  
Recommended Sample Types:  
FFPE    
X
 
Blood (FTA)      
X
Blood (Heparin)
X
X
   
Plant Leaves (FTA)      
X
Plant leaves
X
 X*
   
Buccal cells (FTA)      
X
Buccal cells
X
X
   
Saliva
X
X
   
Tissues
X
 X*
   
Cell pellet
X
X
   
Crude extract
X
X
   
Purified DNA
X
X
   
Polymerase Mix Red Dye Premix Genotyping kit FFPE Kit FTA Card Kit
* The Terra PCR Direct Genotyping Kit includes an extraction buffer, which we recommended using tough plants or tissue samples.

Having trouble with Terra PCR?

Download our revised user manual to see the Visual Troubleshooting guide!
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