Direct PCR allows for amplification directly from the tissue source or crude extracts, without the need for DNA purification. Omitting the multi-step process for preparing genomic DNA helps prevent sample loss, saves time, and reduces cost. Direct PCR vastly simplifies genotyping experiments, especially when the source material is limited.
How is it achieved?
Direct PCR relies on modified polymerases and buffer systems that enable DNA amplification in the presence of PCR inhibitors, growth serum, and other source material components.
What makes our PCR polymerases the best?
Terra PCR Direct mixes and kits are optimized for direct PCR and allow amplification from a variety of sources, without the need for sample preparation. The key is a novel non-Taq DNA polymerase designed specifically to function in non-ideal conditions to amplify templates up to 2kb & 70% GC-content with high yields. All kits come equipped with with hot-start antibody for enhanced specificty.
All mixes and kits are used for amplifying genomic DNA from plant and animal tissue. The polymerase mix contains the Terra PCR polymerase & 2X buffer. The Red Dye Premix has an added gel-loading dye. The genotyping Kit comes complete with tissue extraction buffer & proteinase K. All kits are compatible with “dilution PCR”.
Used to amplify genomic DNA from paraffin-embedded tissues, without deparaffinization or purification steps. Protocol is considered “dilution PCR”.
* In dilution PCR the primary sample is diluted into lysis buffer prior to amplification. Unlike direct PCR, this allows for multiple experiments using a single crude extract.
