What’s your biggest challenge?
Do You Have Trouble with Sample Lysis?
Is Humic Acid Preventing You from Isolating Quality DNA from Soil?
Are You Frustrated with Low Purity and Yield?
The highly variable composition of different soils makes it impossible to maximize DNA yield and purity for all samples with a single lysis buffer. Therefore, NucleoSpin Soil includes two lysis buffers plus an enhancer. You can mix and match these components to obtain the highest yield (2–10 μg DNA, depending on the sample) and purity (A260/280=1.7–1.9) for any type of soil, no matter what humic substances or PCR inhibitors it contains.
Is Your PCR Failing due to PCR Inhibitors?
The only way to be sure that your PCR reactions will be successful is to eliminate PCR inhibitors when you purify DNA from soil. NucleoSpin Soil includes very efficient wash buffers and Inhibitor Removal Columns that eliminate PCR inhibitors. The purified DNA is ready to use for PCR; no dilution or clean-up is necessary. And since the purified DNA does not need to be diluted, your PCR reactions will have maximum sensitivity. NucleoSpin Soil completely removes PCR inhibitors and provides good PCR results from undiluted eluates. DNA was purified with NucleoSpin Soil (using various combinations of the included Lysis Buffers and Enhancer) and with kits from Competitors MP and MO. 2 µl of undiluted eluate from each purification was used as a PCR template with fungi-specific internal transcribed spacer (ITS) primers. NucleoSpin Soil isolated pure DNA from every type of soil, and no dilution was needed for PCR. Competitor MP (Lanes 5) failed to yield DNA pure enough to be used undiluted. PCR from river sediment samples purified with the kit from Competitor MO (Lanes 6) was strongly inhibited. Lane 1. NucleoSpin Soil, using Soil Lysis Buffer SL1. Lane 2. NucleoSpin Soil, using Soil Lysis Buffer SL1 plus Enhancer SX. Lane 3. NucleoSpin Soil, using Soil Lysis Buffer SL2. Lane 4. NucleoSpin Soil, using Soil Lysis Buffer SL2 plus Enhancer SX. Lane 5: Kit from Competitor MP. Lane 6. Kit from Competitor MO.
Simple Soil DNA Purification Protocol
NucleoSpin Soil Specifications
|Format||Mini spin columns|
|Starting Material||<500 mg (wet weight) soil, sludge, or sediment|
|Binding Capacity||50 μg|
|Yield||2–10 μg DNA from soil microbes|
|Elution Volumne||30–100 μl|
|Time||90 min/10 preps|
Visit the Documents tab of the NucleoSpin Soil DNA isolation kit product page to download the User Manual and other documentation.