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Purify Genomic DNA from Environmental Samples with NucleoSpin Soil

Isolate DNA from Soil & Environmental Samples with NucleoSpin Soil

DNA Isolation Kit

Isolate DNA from soil microorganisms.
  • Prevent DNA degradation—NucleoSpin Soil removes humic acid and other PCR inhibitors from soil samples
  • Isolate pure DNA from soil—Use purified DNA directly as a PCR template; no dilution or clean-up necessary

Are you extracting DNA from microbes in soil, sludge, or sediment? We know that this can be challenging, because soil samples vary widely in their pH and the amount and composition of organic and inorganic matter, including PCR inhibitors, polysaccharides, and humic substances. The NucleoSpin Soil DNA isolation kit removes these contaminants, so you can easily detect and quantify microorganisms including Gram-positive and -negative bacteria, archaea, yeast, fungi, and algae.

What’s your biggest challenge?

Do You Have Trouble with Sample Lysis?

Thorough mechanical lysis is an essential first step to break up the cells and spores in soil. Ceramic beads (included with the kit) are the most effective way to perform this initial lysis step.

Is Humic Acid Preventing You from Isolating Quality DNA from Soil?

Are You Frustrated with Low Purity and Yield?

Is Your PCR Failing due to PCR Inhibitors?

Simple Soil DNA Purification Protocol

NucleoSpin Soil Specifications

Technology Silica membrane
Format Mini spin columns
Starting Material <500 mg (wet weight) soil, sludge, or sediment
Binding Capacity 50 μg
Yield 210 μg DNA from soil microbes
Elution Volumne 30100 μl
Time 90 min/10 preps

Visit the Documents tab of the NucleoSpin Soil DNA isolation kit product page to download the User Manual and other documentation.

Cat. # Product Contents Size Price Units Select
740780.10 NucleoSpin® Soil 10 Preps $68.00
740780.50 NucleoSpin® Soil 50 Preps $261.00
740780.250 NucleoSpin® Soil 250 Preps $1,165.00



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