Clontech offers two yellow fluorescent proteins, ZsYellow1 and mBanana.
ZsYellow1 has a true yellow emission, making it ideal for multicolor applications. Like all of Clontech's Living Colors Fluorescent Proteins, ZsYellow1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays (1). The gene for ZsYellow1 has been human codon-optimized to enhance its translation in mammalian cells (2), and adapted for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours).
ZsYellow1 can be used as a molecular tag or as a reporter to visualize, track, and quantify cellular processes including protein synthesis and turnover, protein translocation, gene induction, and cell lineage. Because its excitation and emission spectra are distinct from our other fluorescent proteins, ZsYellow1 can be used for multiplex applications—that is, to simultaneously detect two or more events in the same cell or cell population. For example, it can be used in two- and three- color analyses with AmCyan1 and/or HcRed1. Like all of our Living Colors Fluorescent Proteins, ZsYellow1 is ideal for monitoring gene expression and protein localization in vivo, in situ, and in realtime (3–6).
Figure 1. Four-color visualization of Living Colors Fluorescent Proteins. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated in the same culture dish and imaged at 20X magnification.
Clontech is distributing vectors encoding several of the Fruit Fluorescent Proteins, including mBanana, which were developed in Dr. Roger Tsien’s lab (7–9). The Fruit Fluorescent Proteins are mutants derived from mRFP1, a monomeric mutant of DsRed, by directed mutagenesis (10). They have demonstrated stable expression, perform successfully in numerous fusion applications, and are already well characterized and recognized in the literature.
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