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Cyan Fluorescent Protein Vectors

Cyan Fluorescent Protein Vectors

AmCyan1 can be detected in cells without adding cofactors or substrates, which makes it ideal for use in live cell assays (1). The gene for AmCyan has been human codon-optimized to enhance its translation in mammalian cells (2), and adapted for higher solubility, brighter emission, and rapid chromophore maturation (8–12 hours). AmCyan1 can be used as a molecular tag or as a reporter to visualize, track, and quantify cellular processes including protein synthesis and turnover, protein translocation, gene induction, and cell lineage. Because its excitation and emission spectra are distinct from our other fluorescent proteins, AmCyan1 can be used for multiplex applications—that is, to simultaneously detect two or more events in the same cell or cell population. For example, it can be used in two-, three- and even four-color analyses (Figure 1). Like all of our Living Colors Fluorescent Proteins, AmCyan1 is ideal for monitoring gene expression and protein localization in vivo, in situ, and in realtime (3–6).

HEK 293 cells expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1.

Figure 1. Four-color visualization of Living Colors Fluorescent Proteins. HEK 293 clonal cell lines stably expressing AmCyan1, ZsGreen1, ZsYellow1, or HcRed1 were mixed and plated in the same culture dish and imaged at 20X magnification.

References

  1. Matz, M. V. et al. (1999) Nature Biotechnol. 17(10):969–973. Erratum in: Nature Biotechnol. (1999) 17(12):1227.
  2. Haas, J. et al. (1996) Curr. Biol. 6:315–324.
  3. Chalfie, M. et al. (1994) Science 263:802–805.
  4. Prasher, D. C. et al. (1992) Gene 111:229–233.
  5. Inouye, S. & Tsuji, F. I. (1994) FEBS Letters 341:277–280.
  6. Wang, S. & Hazelrigg, T. (1994) Nature 6479:400–403.

 

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