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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  cDNA_and_Genomic_DNA >  Tissue-Specific_cDNA >  Human_Fetal

Tissue-Specific Human cDNA, Fetal

QUICK-Clone cDNAs are premade double-stranded cDNA from which you can amplify a gene of interest using gene-specific primers. QUICK-Clone cDNA is ideal for amplifying previously isolated, structurally related, or cross-species cDNAs. Through direct amplification of a cDNA of interest, traditional library construction and screening can be avoided. QUICK-Clone cDNA can also be used to generate hybridization probes using gene-specific or degenerate primers (1–5).

A high-quality cDNA template is necessary to obtain good results from PCR amplification. Synthesized from high quality premium Poly A+ RNAs from fetal tissues using an oligo(dT) primer, QUICK-Clone cDNA is purified to remove residual RNA and size-selected to eliminate cDNA fragments smaller than 400 bp. The result is very pure, double-stranded cDNA ready for PCR.

At-A-Glance Documents Images & Data

Features

  • Highly purified double-stranded cDNA
  • Clone genes directly by PCR, rather than library screening
  • Prepared from high-quality human tissues and cell lines
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

Applications

  • Clone cDNAs without library screening
  • Generate hybridization probes using gene-specific or degenerate primers
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

References

  1. Parmentier, M., et al. (1989) Science 246:1620–1622.
  2. Wilks, A. E., et al. (1989) Gene 85:67–74.
  3. Vallins, W. J., et al. (1990) FEBS Letters 270:57–61.
  4. Lee, C. C., et al. (1998) Science 239:1288–1291.
  5. Schuchman, E. H., et al. (1990) Genomics 6:149–158.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
637221 Human Fetal Brain QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637227 Human Fetal Heart QUICK-Clone™ cDNA 2 x 10 Rxns $612.00
637229 Human Fetal Kidney QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637230 Human Fetal Liver QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
 

 

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