QUICK-Clone cDNAs are premade double-stranded cDNA from which you can amplify a gene of interest using gene-specific primers. QUICK-Clone cDNA is ideal for amplifying previously isolated, structurally related, or cross-species cDNAs. Through direct amplification of a cDNA of interest, traditional library construction and screening can be avoided. QUICK-Clone cDNA can also be used to generate hybridization probes using gene-specific or degenerate primers (1–5).
A high-quality cDNA template is necessary to obtain good results from PCR amplification. Synthesized from premium, high quality poly A+ RNA prepared from cancer tissues and various cancer cell lines using an oligo(dT) primer, QUICK-Clone cDNA is purified to remove residual RNA and size-selected to eliminate cDNA fragments smaller than 400 bp. The result is very pure, double-stranded cDNA ready for PCR.